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首页> 外文期刊>Genetic Resources and Crop Evolution >Molecular diversity of Omani wheat revealed by microsatellites: II. Hexaploid landraces
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Molecular diversity of Omani wheat revealed by microsatellites: II. Hexaploid landraces

机译：微卫星揭示的阿曼小麦的分子多样性：II。六倍体地方品种

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摘要

For millennia, wheat (Triticum spp.) has been grown in traditional aflaj-irrigation systems of remote mountain oases in Oman. However, little is known about the diversity of the ancient landraces used. Given recent reports about the occurrence of novel germplasm in such material, the objective of this study was to evaluate the genetic diversity of hexaploid wheat (Triticum aestivum L.) landraces in relation to their geographic origin using microsatellites. The collection covered most of the cultivation areas in northern Oman where wheat landraces are growing. Total genomic DNA was extracted from six pooled plants representing each accession. A total of 161 wheat accessions were assayed using 35 microsatellite loci in which a total of 305 polymorphic bands were recorded for the 35 microsatellites. The polymorphic information content (PIC) across the 35 microsatellite loci ranged from 0.02 to 0.89 with an average of 0.50. A heterozygosity percentage value of 9.09 was determined and the highest level recorded for accessions from the Batinah district. Rare alleles averaged 1.85 with the highest value being from the Dakhilia district. The results indicated a significant correlation between gene diversity and number of alleles across districts. The correlation coefficient between these two variables over the 35 loci was 0.657, whereby correlation coefficients of 0.718, 0.706, 0.657 and 0.651, respectively, were found for the Batinah, Dhahira, Dakhilia and Sharqia materials. Genetic distances indicated that all landraces were closely related. The cluster analysis discriminated most of the landraces accessions. However, it failed to achieve region-specific groupings of landraces. The present study demonstrated the presence of high diversity in Omani landraces and also indicated the effectiveness of microsatellites to describe it.
机译：几千年来，在阿曼偏远山区绿洲的传统黄水灌溉系统中种植了小麦（Triticum spp。）。但是，对于所使用的古代地方品种的多样性知之甚少。鉴于有关这种材料中出现新种质的最新报道，本研究的目的是使用微卫星技术评估六倍体小麦（Triticum aestivum L.）地方品种的遗传多样性与其地理起源的关系。该馆藏涵盖了阿曼北部种植小麦地方品种的大部分耕种地区。从代表每种登录的六种合并植物中提取总基因组DNA。使用35个微卫星基因座分析了总共161种小麦，其中35个微卫星总共记录了305个多态性谱带。 35个微卫星基因座上的多态信息含量（PIC）为0.02至0.89，平均为0.50。确定了9.09的杂合度百分比值，并记录了来自Batinah地区的种质的最高水平。罕见等位基因平均为1.85，其中最高值来自Dakhilia地区。结果表明跨地区基因多样性与等位基因数量之间存在显着相关性。在35个基因座上，这两个变量之间的相关系数为0.657，其中Batinah，Dhahira，Dakhilia和Sharqia材料的相关系数分别为0.718、0.706、0.657和0.651。遗传距离表明所有地方品种都密切相关。聚类分析区分了大多数地方品种。但是，它未能实现特定地区的地方种族分组。本研究证明了阿曼地方种中存在高度多样性，也表明了微卫星对其进行描述的有效性。

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