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首页> 外文期刊>Genetic Testing >Investigation of Association between Plasminogen Activator Inhibitor Type-1 (PAI-1) Gene 4G/5G Polymorphism Frequency and Plasma PAI-1 Enzyme Activity in Patients with Acute Stroke
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Investigation of Association between Plasminogen Activator Inhibitor Type-1 (PAI-1) Gene 4G/5G Polymorphism Frequency and Plasma PAI-1 Enzyme Activity in Patients with Acute Stroke

机译：急性脑卒中患者血浆纤溶酶原激活物抑制剂1型（PAI-1）基因4G / 5G多态性频率与血浆PAI-1酶活性之间的相关性研究

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Aim: This study was carried out to determine if there is any association between plasminogen activator inhibitor type-1 (PAI-1) gene 4G/5G polymorphism and plasma PAI-1 enzyme activity in acute stroke patients. Methods: In this study, 333 genomic DNAs (from 253 acute stroke patients and 80 healthy subjects) were analyzed. Genomic DNAs were prepared from peripheral blood using a saline method. These DNAs were amplified by PCR method using primers specific for 4G and 5G alleles. PCR products were separated by 2% agarose gel electrophoresis and visualized by a charge coupled device (CCD) camera. PAI-1 enzyme activities were measured by ELISA method. The results were evaluated statistically with Student's t-test, X~2-test, one-way analysis of variance, and stepwise regression analysis. Results: In this study, frequency of PAI-1 gene 4G5G genotype was found to be low both in patients and controls. PAI-1 enzyme activities were significantly increased in acute stroke patients compared to controls. Although PAI-1 gene 4G5G genotype frequencies were low, the patients carrying this allele had highest plasma PAI-1 enzyme activity; likewise, although PAI-1 gene 4G4G genotype frequencies were high, the patients carrying this allele had lowest plasma PAI-1 enzyme activities. Homocysteine levels had a positive effect of 65% on plasma PAI-1 enzyme activities. Conclusion: Consequently, in this study, we may assert that PAI-1 gene, 4G4G and 5G5G genotypes, PAI-1 activity, and homocysteine level determination are significant criteria for identifying patients who are likely to develop stroke; on the other hand, a direct relation does not exist between gene polymorphism and enzyme activity.
机译：目的：本研究旨在确定急性脑卒中患者中纤溶酶原激活物抑制剂1型（PAI-1）基因4G / 5G多态性与血浆PAI-1酶活性之间是否存在关联。方法：在这项研究中，分析了333个基因组DNA（来自253名急性中风患者和80名健康受试者）。使用盐水法从外周血制备基因组DNA。使用对4G和5G等位基因具有特异性的引物，通过PCR方法扩增这些DNA。 PCR产物通过2％琼脂糖凝胶电泳分离，并通过电荷耦合器件（CCD）相机可视化。通过ELISA法测量PAI-1酶活性。通过学生t检验，X〜2检验，方差单向分析和逐步回归分析对结果进行统计学评估。结果：在这项研究中，发现患者和对照中PAI-1基因4G5G基因型的频率均较低。与对照组相比，急性中风患者的PAI-1酶活性显着增加。尽管PAI-1基因4G5G基因型频率较低，但携带该等位基因的患者血浆PAI-1酶活性最高。同样，尽管PAI-1基因4G4G基因型频率很高，但携带该等位基因的患者血浆PAI-1酶活性最低。同型半胱氨酸水平对血浆PAI-1酶活性有65％的积极影响。结论：因此，在这项研究中，我们可以断言PAI-1基因，4G4G和5G5G基因型，PAI-1活性和同型半胱氨酸水平的确定是鉴定可能发展为中风的患者的重要标准。另一方面，基因多态性与酶活性之间不存在直接关系。

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