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首页> 外文期刊>Genetica >Cryptic clonal lineages and genetic diversity in the loach Misgurnus anguillicaudatus (Teleostei: Cobitidae) inferred from nuclear and mitochondrial DNA analyses
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Cryptic clonal lineages and genetic diversity in the loach Misgurnus anguillicaudatus (Teleostei: Cobitidae) inferred from nuclear and mitochondrial DNA analyses

机译：从核和线粒体DNA分析推断出泥MiMisgurnus anguillicaudatus（Teleostei：Cobitidae）的隐性克隆谱系和遗传多样性

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摘要

In the loach Misgurnus anguillicaudatue, the asexual lineage, which produces unreduced clonal diploid eggs, has been identified. Among 833 specimens collected from 54 localities in Japan and two localities in China, 82 candidates of other lineage(s) of cryptic clones were screened by examining RFLP (restriction fragment length polymorphism)-PCR haplotypes in the control region of mtDNA. This analysis was performed because triploid loaches arise from the accidental incorporation of the sperm nucleus into unreduced diploid eggs of a clone. The categorization of members belonging to three newly identified lineages (clones 2–4) and the previously identified clonal lineage (clone 1) was verified by evaluating the genetic identity between two or more individuals from each clonal lineage based on RAPD (random amplified polymorphic DNA)-PCR and multilocus DNA fingerprints. We detected 75 haplotypes by observing the nucleotide status at variable sites from the control region of mtDNA. Phylogenic trees constructed from such sequences showed two highly diversified clades, A and B, that were beyond the level common for interspecific genetic differentiation. That result suggests that M. anguillicaudatus in Japan is not a single species entity. Two clone-specific mtDNA sequences were included in clade A, and the loaches with such sequences may be the maternal origin of the clones.
机译：在泥MiMisgurnus anguillicaudatue中，已鉴定出无性谱系，该谱系产生未减少的克隆二倍体卵。在来自日本54个地区和中国两个地区的833个标本中，通过检查mtDNA控制区域的RFLP（限制性片段长度多态性）-PCR单倍型，筛选了其他82个其他隐性克隆候选者。进行此分析是因为三倍体泥ache是​​由于精子核偶然掺入克隆的未还原二倍体卵而引起的。通过评估基于RAPD（随机扩增的多态性DNA）的每个克隆谱系中两个或多个个体之间的遗传同一性，验证了属于三个新鉴定的谱系（克隆2-4）和先前鉴定的克隆谱系（克隆1）的成员的分类。 ）-PCR和多基因座DNA指纹图谱。我们通过观察mtDNA控制区域可变位点的核苷酸状态来检测75种单倍型。由此类序列构建的系统发育树显示出两个高度多样化的进化枝，A和B，超出了种间遗传分化的通用水平。该结果表明，日本的M. anguillicaudatus不是单个物种的实体。进化枝A中包括两个克隆特异性mtDNA序列，并且具有此类序列的泥ache可能是克隆的母源。

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