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首页> 外文期刊>Genetics >Identification of an Alternative Oxidase Induction Motif in the Promoter Region of the aod-1 Gene in Neurospora crassa
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Identification of an Alternative Oxidase Induction Motif in the Promoter Region of the aod-1 Gene in Neurospora crassa

机译：鉴定神经孢菌中aod-1基因启动子区域的另一种氧化酶诱导基序

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The nuclear aod-1 gene of Neurospora crassa encodes the alternative oxidase and is induced when thenstandard cytochrome-mediated respiratory chain of mitochondria is inhibited. To study elements of thenpathway responsible for alternative oxidase induction, we generated a series of mutations in the regionnupstream from the aod-1 structural gene and transformed the constructs into an aod-1 mutant strain.nTransformed conidia were plated on media containing antimycin A, which inhibits the cytochromemediatednelectron transport chain so that only cells expressing alternative oxidase will grow. Using thisnfunctional in vivo assay, we identified an alternative oxidase induction motif (AIM) that is required fornefficient expression of aod-1. The AIM sequence consists of two CGG repeats separated by 7 bp and isnsimilar to sequences known to be bound by members of the Zn(II)2Cys6 binuclear cluster family ofntranscription factors. The AIM motif appears to be conserved in other species found in the ordernSordariales.
机译：粗糙神经孢菌的核aod-1基因编码另一种氧化酶，然后在抑制标准的细胞色素介导的线粒体呼吸链时被诱导。为了研究负责替代氧化酶诱导的途径途径，我们从aod-1结构基因的上游区域产生了一系列突变，并将构建体转化为aod-1突变菌株。将转化的分生孢子接种在含有抗霉素A的培养基上抑制细胞色素介导的电子传输链，因此只有表达替代氧化酶的细胞才能生长。使用此功能的体内分析，我们确定了aod-1无效表达所需的另一种氧化酶诱导基序（AIM）。 AIM序列由两个CGG重复序列组成，它们间隔7 bp，与已知被转录因子Zn（II）2Cys6双核簇家族成员结合的序列相似。 AIM基序似乎在Sordariales目中发现的其他物种中是保守的。

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