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Environmental factors influencing the distribution and abundance of Alexandrium catenella in Kachemak bay and lower cook inlet, Alaska

机译:环境因素影响阿拉斯加Kachemak海湾和下厨区入口处亚历山大连叶藻的分布和丰度

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Despite the long history of paralytic shellfish poisoning (PSP) events in Alaska, little is known about the seasonal distribution and abundance of the causative organism, Alexandrium, or the environmental factors that govern toxic bloom development. To address this issue, a five year study (2012-2017) was undertaken in Kachemak Bay and lower Cook Inlet Alaska to determine how the occurrence of Alexandrium catenella, the dominant PSPcausing Alexandrium species, was influenced by temperature, salinity, nutrient concentrations, and other environmental factors. Cell concentrations from 572 surface water samples were estimated using quantitative PCR. Monthly sampling revealed a seasonal pattern of A. catenella bloom development that was positively correlated with water temperature. Prevailing salinity conditions did not significantly affect abundance, nor was nutrient limitation a direct factor. Elevated cell concentrations were detected in 35 samples from Kachemak Bay (100-3050 cell eq. L-1) while a maximum abundance of 67 cell eq. L-1 was detected in samples from lower Cook Inlet sites. Monitoring data showed average water temperatures in Kachemak Bay increased by similar to 2 degrees C over the course of the study and were accompanied by an increase in Alexandrium abundance. Based on these findings, 7-8 degrees C appears to represent a temperature threshold for significant bloom development in Kachemak Bay, with the greatest risk of shellfish toxicity occurring when temperatures exceed 10-12 degrees C. The role of temperature is further supported by time series data from the Alaska Coastal Current (station GAK1), which showed that summertime shellfish toxicity events in Kachemak Bay generally followed periods of anomalously high winter water temperatures. These data indicate monitoring changes in water temperatures may be used as an early warning signal for subsequent development of shellfish toxicity in Kachemak Bay.
机译:尽管阿拉斯加的麻痹性贝类中毒(PSP)事件历史悠久,但对引起病原体亚历山大藻的季节性分布和丰富程度或控制有毒花开发育的环境因素知之甚少。为了解决这个问题,在Kachemak湾和阿拉斯加下游库克湾(Cook Inlet Alaska)进行了为期五年的研究(2012-2017),以确定温度,盐度,养分浓度和其他环境因素。使用定量PCR估算了572个地表水样品中的细胞浓度。每月采样显示,链状芽孢杆菌花开发展的季节性模式与水温呈正相关。普遍的盐度条件并没有显着影响丰度,养分限制也不是直接因素。在来自Kachemak湾的35个样品(100-3050个细胞当量的L-1)中检测到细胞浓度升高,最大丰度为67个细胞当量。在库克湾下游地区的样品中检测到L-1。监测数据显示,在研究过程中,Kachemak湾的平均水温升高了约2摄氏度,并伴随着亚历山大丰度的提高。根据这些发现,7-8摄氏度似乎代表着凯赫迈克湾显着开花发展的温度阈值,当温度超过10-12摄氏度时,发生贝类毒性的风险最大。温度的作用得到时间的进一步支持来自阿拉斯加沿海水流(GAK1站)的系列数据表明,Kachemak湾的夏季贝类毒性事件通常是在冬季水温异常升高之后进行的。这些数据表明,监测水温的变化可能被用作预警信号,以指示随后在Kachemak湾发生贝类毒性。

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