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Alexandrium diversaporum sp. nov., a new non-saxitoxin producing species: Phylogeny, morphology and sxtA genes

机译:亚历山大草十一月,一个新的非萨克斯毒素产生物种:系统发育,形态学和sxtA基因

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摘要

Species of the PST producing planktonic marine dinoflagellate genus Alexandrium have been intensively scrutinised, and it is therefore surprising that new taxa can still be found. Here we report a new species, Alexandrium diversaporum nov. sp., isolated from spherical cysts found at two sites in Tasmania, Australia. This species differs in its morphology from all previously reported Alexandrium species, possessing a unique combination of morphological features: the presence of 2 size classes of thecal pores on the cell surface, a medium cell size, the size and shape of the 6", 1', 2"" and Sp plates, the lack of a ventral pore, a lack of anterior and posterior connecting pores, and a lack of chain formation. We determined the relationship of the two strains to other species of Alexandrium based on an alignment of concatenated SSU-ITS1, 5.8S, ITS2 and partial LSU ribosomal RNA sequences, and found A. diversaporum to be a sister group to Alexandrium leei with high support. A. leei shares several morphological features, including the relative size and shapes of the 6", 1', 2"" and Sp plates and the fact that some strains of A leei have two size classes of thecal pores. We examined A. diversaporum strains for saxitoxin production and found them to be non-toxic. The species lacked sequences for the domain A4 of sxtA, as has been previously found for non-saxitoxin producing species of Alexandrium.
机译:生产PST的浮游性海洋鞭毛藻亚历山大藻属的物种已受到严格审查,因此令人惊讶的是仍然可以找到新的分类单元。在这里,我们报告一个新物种,亚历山大草novsaporum nov。从澳大利亚塔斯马尼亚州两个地点发现的球形囊肿中分离得到的小球藻。该物种的形态不同于以前报道的所有亚历山大属物种,具有独特的形态特征组合:细胞表面存在2种大小的鞘孔,中等细胞大小,6“,1的大小和形状',2“”和Sp板,腹侧孔的缺失,前后孔的缺失以及链的形成。我们根据的排列确定了这两种菌株与亚历山大藻其他种的关系。将SSU-ITS1、5.8S,ITS2和部分LSU核糖体RNA序列连接起来,发现杂色曲霉是亚历山大亚历山大草的姐妹群,并得到了很高的支持,雷伊草共有几个形态特征,包括6个的相对大小和形状。 “,1',2”“和Sp板,以及某些A leei菌株具有两个大小类别的鞘孔这一事实。我们检查了A. diversaporum菌株产生的毒素的含量,发现它们是无毒的。该物种缺少sxtA域A4的序列,这是以前发现的非萨克毒素产生物种亚历山大草的序列。

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  • 来源
    《Harmful Algae》 |2014年第1期|54-65|共12页
  • 作者单位

    Sydney Institute of Marine Science, Chowder Bay Road, Mosman, NSW, Australia,School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia,Plant Functional Biology and Climate Change Cluster, University of Technology, Sydney, Australia;

    Senckenberg Research Institute, Senckenberg am Meer, German Center for Marine Biodiversity Research (DZMB), Suedstrand 44, D-26382 Wilhelmshaven, Germany;

    Microbial Evolution Research Group, Department of Biosciences, University of Oslo, 0316 Oslo, Norway;

    National Centre for Marine Conservation and Resource Sustainability, Australian Maritime College, University of Tasmania, Locked Bag 1370, Launceston, Tasmania 7250, Australia;

    Section Ecological Chemistry, Alfred-Wegener-Institut Helmholtz-Zentrum fuer Polar- und Meeresforschung, Bremerhaven, Germany;

    Sydney Institute of Marine Science, Chowder Bay Road, Mosman, NSW, Australia;

    Sydney Institute of Marine Science, Chowder Bay Road, Mosman, NSW, Australia;

    Cawthron Institute, Private Bag 2, Nelson 7042, New Zealand;

    Tasmanian Herbarium, University of Tasmania, Private Bag 4, Hobart, Tasmania 7001, Australia;

    School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia;

    Institute for Marine and Antarctic Studies, University of Tasmania, Private Bag 55, Hobart, Tasmania 7001, Australia;

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  • 正文语种 eng
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  • 关键词

    Alexandrium diversaporum; Phylogenetics; PSP; PST; Saxitoxin; sxtA; Systematics;

    机译:亚历山大草;系统发育学;PSP;太平洋标准时间;毒素sxtA;系统学;

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