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首页> 外文期刊>Harmful Algae >DNA barcoding species in Alexandrium tamarense complex using ITS and proposing designation of five species
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DNA barcoding species in Alexandrium tamarense complex using ITS and proposing designation of five species

机译：利用ITS技术对塔玛亚历山大藻复合体中的DNA进行条形码编码，并提议指定5种

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摘要

Alexandrium species can be very difficult to identify, with A catenella, A. tamarense, and A. fundyense that compose "Alexandrium tamarense species complex" (Atama complex) as a distinct example. DNA barcoding is promising to offer a solution but remains to be established. In this study, we examined the utility of ITS in resolving the Atama species complex, by analyzing previously studied strains plus unstudied Chinese strains within the LSU- and SSU-Rdna based group/clade frameworks recently established. We further investigated the presence of intragenomic polymorphism and its implications in species delimitation. Similar to the previous SSU and LSU results, our ITS-based phytogenies divided the complex to five clusters, but with longer and evener branch lengths between the clusters. Based on the ITS region, the inter-cluster genetic distances (p = 0.134-0.216) were consistently and substantially greater than intra-cluster genetic distances (p = 0.000-0.066), with an average inter-cluster (species) distance (p = 0.167) 7.6-fold of the average intraspecific difference (p = 0.022), qualifying the approximately 510-520 bp ITS as a DNA barcode for Atama complex. We detected varying levels of intragenomic polymorphism in ITS but found that this did not impact the taxon-resolving power of this gene. With this DNA barcode, the new East and South China Sea strains and one Antarctic strain were placed in Clade IIC/Group Ⅳ, even though there were 7-10 polymorphic sites in their ITS, in contrast to none in SSU. Furthermore, our results suggest that the five clusters are recognizable as distinct species according to the phylogenetic species concept. Based on the phylogenetic placements of the type-locality strains of the existing three morphospecies and the dominant localities of other strains, we propose that Group Ⅰ/Clade I be designated as A. fundyense, Group Ⅲ/Clade IIB as A. tamarense, Group Ⅳ/Clade IIC as A catenella, Group Ⅱ/Clade IIA as A. mediterranis, and Group Ⅴ/Clade IID as A australis.
机译：亚历山大藻物种可能很难辨认，例如，组成“亚历山大亚历山大藻tamarense物种复合体”（阿塔玛复合体）的A连体毛虫，塔玛氏菌和粉虱。 DNA条形码有望提供解决方案，但仍有待建立。在这项研究中，我们通过分析先前研究的菌株以及最近建立的基于LSU-和SSU-Rdna的群体/进化框架中未研究的中国菌株，研究了ITS在解决Atama物种复合物中的效用。我们进一步调查了基因组内多态性的存在及其对物种划界的影响。与以前的SSU和LSU结果相似，我们基于ITS的植物遗传学将复合物分为五个簇，但簇之间的分支长度更长或更均匀。根据ITS区域，集群间的遗传距离（p = 0.134-0.216）始终大于集群内的遗传距离（p = 0.000-0.066），并且平均集群间（物种）距离（p = 0.167）种内平均差异的7.6倍（p = 0.022），使大约510-520 bp ITS符合Atama复合物的DNA条码。我们在ITS中检测到不同水平的基因组内多态性，但发现这不会影响该基因的分类单元分辨能力。用这种DNA条形码，即使在其ITS中有7-10个多态性位点，新的东海和南海菌株和一个南极菌株也被置于克莱德IIC /Ⅳ组，而在SSU中则没有。此外，我们的研究结果表明，根据系统发生物种概念，这五个簇可以识别为不同的物种。根据现有3个形态种的类型-地方性菌株的系统发育位置和其他菌株的优势部位，我们建议将Ⅰ类/进化枝I指定为A.fundyense，将Ⅲ/ Clade IIB命名为A. tamarense，Group。 Ⅳ/ Clade IIC为叶形虫，Ⅱ/ Clade IIA为中叶土壤杆菌，Ⅴ/ Clade IID为澳大利亚。

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  • 来源
    《Harmful Algae》 |2014年第1期|100-113|共14页
  • 作者

    Lu Wang; Yunyun Zhuang; Huan Zhang; Xin Lin; Senjie Lin;

  • 作者单位

    Marine Biodiversity and Global Change Research Center and State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China;

    Department of Marine Sciences, University of Connecticut, Groton, CT 06340, USA;

    Department of Marine Sciences, University of Connecticut, Groton, CT 06340, USA;

    Marine Biodiversity and Global Change Research Center and State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China;

    Marine Biodiversity and Global Change Research Center and State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China,Department of Marine Sciences, University of Connecticut, Groton, CT 06340, USA;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Alexandrium tamarense complex; ITS rDNA; DNA barcode; Polymorphism; Genetic distance; Phylogeny;

    机译：亚历山大他玛伦山综合体;ITS rDNA;DNA条形码多态性遗传距离系统发育;

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