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首页> 外文期刊>Harmful Algae >Monitoring a toxic bloom of Alexandrium minutum using novel microarray and multiplex surface plasmon resonance biosensor technology
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Monitoring a toxic bloom of Alexandrium minutum using novel microarray and multiplex surface plasmon resonance biosensor technology

机译：使用新型微阵列和多重表面等离振子共振生物传感器技术监测亚历山大草的有毒花朵

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摘要

Blooms of Alexandrium occur annually during the summer months in the North Channel of Cork Harbour on the south coast of Ireland. This study monitored an extensive bloom of the toxin producing Alexandrium minutum during the summer of 2011 with the use of the MIDTAL (Microarrays for the Detection of Toxic Algae) microarray and a prototype multiplex surface plasmon resonance (multi SPR) biosensor. Microarray signal intensities and toxin results from three testing platforms of the prototype multi SPR biosensor, commercial (CER) enzyme-linked immunosorbent assay (ELISA) and high performance liquid chromatography (HPLC) were compared against light microscopy counts. The main aim was to demonstrate the use of these methodologies to support national monitoring agencies by providing a faster and more accurate means of identifying and quantifying the harmful phytoplankton community and their toxins in natural water samples. Both the microarray signals and multi SPR biosensor results followed a significant trend with light microscopy results and both techniques indicated detection limits of ＜4000 cells of A minutum in natural seawater samples.
机译：夏季，每年夏季，爱尔兰南部海岸的科克港北海道都会发生亚历山大花的绽放。这项研究通过使用MIDTAL（用于检测有毒藻类的微阵列）微阵列和原型多重表面等离振子共振（Multi SPR）生物传感器，监测了在2011年夏季产生毒素的亚历山大藻的大量繁殖。比较了原型多SPR生物传感器，商业（CER）酶联免疫吸附测定（ELISA）和高效液相色谱（HPLC）的三个测试平台的微阵列信号强度和毒素结果与光学显微镜计数的比较。主要目的是通过提供一种更快，更准确的方法来识别和量化自然水样中有害的浮游植物群落及其毒素，来证明使用这些方法来支持国家监测机构。微阵列信号和多SPR生物传感器结果均呈现出光学显微镜结果的显着趋势，两种技术均表明天然海水样品中A minutum的＜4000个细胞的检出限。

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  • 来源
    《Harmful Algae》 |2014年第2期|40-48|共9页
  • 作者

    Gary R. McCoy; Sara McNamee; Katrina Campbell; Christopher T. Elliott; Gerard T.A. Fleming; Robin Raine;

  • 作者单位

    The Ryan Institute, School of Natural Sciences (Microbiology), National University of Ireland, Galway, Ireland;

    Institute for Global Food Security, School of Biological Sciences, Queen's University, Stranmillis Road, Belfast, UK;

    Institute for Global Food Security, School of Biological Sciences, Queen's University, Stranmillis Road, Belfast, UK;

    Institute for Global Food Security, School of Biological Sciences, Queen's University, Stranmillis Road, Belfast, UK;

    The Ryan Institute, School of Natural Sciences (Microbiology), National University of Ireland, Galway, Ireland;

    The Ryan Institute, National University of Ireland, Galway, Ireland;

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  • 正文语种 eng
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  • 关键词

    Alexandrium; Biosensor; Microarray; MIDTAL; RNA; Saxitoxins;

    机译：亚历山大;生物传感器微阵列;MIDTAL;RNA;毒素;

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