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psbA based molecular analysis of cross-feeding experiments suggests that Dinophysis acuta does not harbour permanent plastids

机译:基于psbA的交叉进料实验的分子分析表明,Dinophysis acuta不包含永久质体

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摘要

Toxic marine dinoflagellate species of the genus Dinophysis Ehrenberg are obligate mixotrophs that require feeding on the ciliate Mesodinium rubrum and light to achieve growth. It is now well known that they harbour plastids of cryptophyte origin, particularly of the genus Teleaulax, Plagioselmis or Geminigera group (TPG clade). Nevertheless, whether these plastids are permanent, or periodically acquired from M. rubrum prey, need additional studies in different phototrophic Dinophysis species. The origin of plastids from Dinophysis acuta Ehrenberg, one of the main agents of diarrhetic shellfish poisoning (DSP) outbreaks in Western Europe, was investigated here. Cross feeding-starvation experiments were carried out with cultures of D. acuta using M. rubrum as prey, the latter fed with two cryptophyte species, Teieaulax amphioxeia Hill and Teleaulax gracilis, belonging to the TPG clade in addition to Falcomonas sp. and Hemiselmis sp. The fate of cryptophyte plastids transferred to D. acuta through its ciliate prey was investigated using the plastid psbA gene as a tracer.
机译:Dinophysis Ehrenberg属的有毒海洋鞭毛藻物种是专性的混合营养生物,需要以纤毛中生红球菌和轻食为食,以实现生长。现在众所周知,它们具有隐生植物来源的质体,特别是Teleaulax,Plagioselmis或Geminigera类(TPG进化枝)。然而,无论这些质体是永久性的,还是周期性地从红僵菌的猎物中获取,都需要在不同的光养性Dinophysis物种中进行进一步的研究。在这里研究了西欧腹泻性贝类中毒(DSP)暴发的主要病因之一——Dinophysis acuta Ehrenberg的质体来源。用红毛丹毒杆菌作为猎物,对阿氏a(D。acuta)的培养物进行交叉喂养饥饿实验,后者喂食了两种隐藻类物种,即Teieaulax amphioxeia Hill和Teleaulax gracilis,除了Falcomonas sp。以外还属于TPG进化枝。和Hemiselmis sp。通过使用质体psbA基因作为示踪剂,研究了通过其纤毛虫转移到A. acuta的隐生植物质体的去向。

著录项

  • 来源
    《Harmful Algae》 |2014年第5期|20-28|共9页
  • 作者单位

    Departamento de Biologia Molecular, Universidad Autonoma de Madrid (UAM), Cantoblanco, 28049 Madrid, Spain;

    Laboratorio de Control de Calidad de los Recursos Pesqueros, Consejeria de Agriculturay Pesca, Junta de Andalucia, Agenda de Gestion Agraria y Pesquera de Andalucia (AGAPA), Spain;

    Laboratorio de Control de Calidad de los Recursos Pesqueros, Consejeria de Agriculturay Pesca, Junta de Andalucia, Agenda de Gestion Agraria y Pesquera de Andalucia (AGAPA), Spain;

    Instituto Espanol de Oceanografia (IEO), Centro Oceanografico de Vigo, Subida a Radiofaro 50, 36390 Vigo, Spain;

    Departamento de Biologia Molecular, Universidad Autonoma de Madrid (UAM), Cantoblanco, 28049 Madrid, Spain;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Cryptophytes; Dinophysis acuta; Mesodinium rubrum; PCR; Plastids; psbA; RFLPs;

    机译:隐植物;龙眼恐龙中生红麻;PCR;质体;psbA;RFLP;

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