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Distribution and molecular phylogeny of the dinoflagellate genus Ostreopsis in Thailand

机译:泰国鞭毛鞭毛藻属的分布和分子系统发育

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摘要

The toxic benthic dinoflagellate genus Ostreopsis has been connected to the production of palytoxin and its analogs in many tropical and temperate areas. Although the type species, O. siamensis, was originally described from the Gulf of Thailand in 1901, little is known about the species composition and distribution of the genus Ostreopsis in Thailand. In this study, a total of 64 Ostreopsis strains isolated from the Andaman Sea as well as the Gulf ofThailand were investigated by analyzing the nucleotide sequences of the LSU rDNA D1/D2, D8/D10 and ITS-5.8S rDNA regions. Phylogenetic analyses (BI and ML) resulted in some of the strains being assigned to previously described clades, O. cf. ovata and Ostreopsis sp. 6, and revealed the existence of a novel clade named Ostreopsis sp. 7, which exhibited large genetic distances from the other clades. Among O. cf. ovata, several strains from Thailand were formed into a new subclade, the Thailand subclade, whereas a few strains belonged to the South China Sea subclade. Morphometric characteristics such as the cell sizes of the two O. cf. ovata subclades and those of Ostreopsis sp. 7 were not significantly different from each other (p > 0.05). Their characteristics were similar but slightly different from those of O. ovata and were significantly different from those of Ostreopsis sp. 6 (p < 0.05). Toxicities of Ostreopsis from Thailand were evaluated using mouse bioassay. Strains of Ostreopsis sp. 6 and Ostreopsis sp. 7 tested were highly toxic, while the two subclades of O. cf. ovata strains seemed to be nontoxic. This study suggests that toxic Ostreopsis sp. 7 is distributed in the Andaman Sea, whereas the two subclades of O. cf. ovata and toxic Ostreopsis sp. 6 are distributed in the Gulf of Thailand.
机译:在许多热带和温带地区,有毒的底栖鞭毛鞭毛藻属Ostreopsis已与产生草毒素及其类似物有关。尽管O. siamensis类型种最初是在1901年从泰国湾描述的,但对泰国Ostreopsis属的物种组成和分布知之甚少。在这项研究中,通过分析LSU rDNA D1 / D2,D8 / D10和ITS-5.8S rDNA区域的核苷酸序列,共调查了从安达曼海和泰国湾分离出的64种骨质疏松菌株。系统发育分析(BI和ML)导致某些菌株被分配到先前描述的进化枝上。卵和骨质疏松症6,并揭示了一个名为Ostreopsis sp。的新型进化枝的存在。图7显示出与其他进化枝的遗传距离较远。在O. cf.卵子,一些来自泰国的菌株形成了一个新的子壳,泰国子壳,而一些菌株属于南中国海子壳。形态特征,例如两个O的像元大小。卵子子窝和Ostreopsis sp。 7个彼此之间无显着差异(p> 0.05)。它们的特征相似,但是与O. ovata的特征略有不同,并且与Ostreopsis sp。的特征明显不同。 6(p <0.05)。使用小鼠生物测定法评估了泰国骨质疏松症的毒性。菌株Ostreopsis sp。 6和Ostreopsis sp。测试的7个剧毒,而O. cf.的两个小部分卵形菌株似乎无毒。这项研究表明,有毒的骨质疏松症。 7个散布在安达曼海,而O.cf.的两个子卵和有毒的骨质疏松症6个散布在泰国湾。

著录项

  • 来源
    《Harmful Algae》 |2014年第7期|160-171|共12页
  • 作者单位

    LAQUES (Laboratory of Aquatic Environmental Science), Faculty of Agriculture, Kochi University, Kochi 783-8502, Japan,The United Graduate School of Agricultural Sciences, Ehime University, Ehime 790-8566, Japan;

    LAQUES (Laboratory of Aquatic Environmental Science), Faculty of Agriculture, Kochi University, Kochi 783-8502, Japan,The United Graduate School of Agricultural Sciences, Ehime University, Ehime 790-8566, Japan;

    LAQUES (Laboratory of Aquatic Environmental Science), Faculty of Agriculture, Kochi University, Kochi 783-8502, Japan;

    Fukui Prefectural University, 1-1 Gakuen-cho, Obama City, Fukui 917-0003, Japan;

    LAQUES (Laboratory of Aquatic Environmental Science), Faculty of Agriculture, Kochi University, Kochi 783-8502, Japan;

    LAQUES (Laboratory of Aquatic Environmental Science), Faculty of Agriculture, Kochi University, Kochi 783-8502, Japan;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Ostreopsis; Benthic dinoflagellate; Distribution; Phylogeny; Thailand; Toxicity;

    机译:骨质疏松症;底鞭毛鞭毛;分配;系统发育泰国;毒性;

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