首页>
外国专利>
Molecular identification method for single dinoflagellate cyst
Molecular identification method for single dinoflagellate cyst
展开▼
机译：单鞭毛藻囊肿的分子鉴定方法
展开▼
页面导航
摘要
著录项
相似文献
摘要
The present invention provides a molecular identification method for a single dinoflagellate cyst, including: obtaining crushed liquids of the single dinoflagellate cyst; performing a first PCR amplification with the crushed liquids as a template, SEQ ID NO: 1 (GTCCGCCCTCTGGGTG) as a forward primer, and SEQ ID NO: 2 (TCGCAGTAGTSYGTCTTTAAC) as a reverse primer to obtain an amplification product so as to determine the species of the dinoflagellate cyst. If the first amplification product is unsuitable for sequencing due to weak bands by electrophoresis detection, a second (nested) PCR amplification and sequencing is performed with SEQ ID NO: 1 (GTCCGCCCTCTGGGTG) as a forward primer and SEQ ID NO: 2 (TCGCAGTAGTSYGTCTTTAAC) as a reverse primer to determine the species of dinoflagellate.
展开▼
机译：本发明提供了一种单鞭毛囊肿的分子鉴定方法，包括：获得单鞭毛囊肿的压碎液;以粉碎液为模板，以SEQ ID NO：1（GTCCGCCCTCTGTGTGTG）为正向引物，以SEQ ID NO：2（TCGCAGTAGTSYGTCTTTAAC）为反引物进行第一次PCR扩增，从而获得扩增产物，以确定其种类鞭毛囊肿。如果第一个扩增产物由于电泳检测弱条带而不适用于测序，则以SEQ ID NO：1（GTCCGCCCTCTGGGTGTG）作为正向引物和SEQ ID NO：2（TCGCAGTAGTSYGTCTTTAAC）进行第二次（嵌套）PCR扩增和测序作为确定底鞭毛藻物种的反向引物。
展开▼
