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A feeding study to probe the uptake of Maitotoxin by snapper (Pagrus auratus)

机译:一项喂养研究,以探讨鲷鱼(Pagrus auratus)对Maitotox的吸收

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摘要

The role of ciguatoxins (CTX) in Ciguatera fish poisoning (CFP) has been investigated previously, but little is known about Maitotoxin (MTX) and whether it plays a significant role in causing human illness. The MTXs are known to have slightly higher potency than CTXs when administered intraperitoneally in mice, but are less potent when administered orally, due to their water solubility. It is not known whether MTXs accumulate in carnivorous fish tissues, and to investigate this further fish feeding trials with snapper (Pagrus auratus) were undertaken. Replicate P. auratus were fed with juvenile mullet (Aldrichetta forsteri) injected with a pellet of a known quantity of Gambierdiscus australes, which is a known producer of MTX. The levels of MTX in different fish tissues were determined using two newly developed sensitive LC-MS/ MS assays for MTX that monitor either a specific cleavage fragment (generated from micro-scale oxidation of the intact toxin) or the intact toxin itself. The investigations revealed the presence of MTX in P. auratus viscera, liver and muscle samples. The presence of Gambierdiscus-related genes in P. auratus digestive organs was confirmed using DNA amplification technology.
机译:先前已经研究了瓜瓜毒素(CTX)在瓜瓜鱼中毒(CFP)中的作用,但对麦毒素(MTX)及其在导致人类疾病中起重要作用的了解甚少。已知当在小鼠腹膜内给药时,MTX的效力比CTX略高,但是由于其水溶性,口服时的效力较低。尚不清楚MTX是否在肉食性鱼类组织中蓄积,并进行了进一步研究,用鲷鱼(Pagrus auratus)进行鱼类饲养试验。用复制量已知的MTX产生者的已知量的南方冈比亚球团的小鱼(Aldrichetta forsteri)喂食复制的体育金鱼。使用两种新开发的针对MTX的灵敏LC-MS / MS分析法测定不同鱼组织中MTX的水平,该方法可监测特定的裂解片段(从完整毒素的微尺度氧化产生)或完整毒素本身。调查显示内脏金黄色葡萄球菌,肝脏和肌肉样本中存在MTX。使用DNA扩增技术证实了金黄色葡萄球菌消化器官中与甘比氏菌相关的基因的存在。

著录项

  • 来源
    《Harmful Algae》 |2014年第7期|125-132|共8页
  • 作者单位

    School of Biotechnology & Biomolecular Sciences, University of New South Wales, Kensington, NSW 2052, Australia,Plant Functional Biology & Climate Change Cluster, University Of Technology Sydney, Ultimo, NSW 2007, Australia;

    Cawthron Institute, 98 Halifax Street, Private Bag 2, Nelson 7010, New Zealand;

    Cawthron Institute, 98 Halifax Street, Private Bag 2, Nelson 7010, New Zealand;

    Cawthron Institute, 98 Halifax Street, Private Bag 2, Nelson 7010, New Zealand;

    Cawthron Institute, 98 Halifax Street, Private Bag 2, Nelson 7010, New Zealand;

    The New Zealand Institute for Plant & Food Research Limited, Plant & Food Research Nelson, P.O. Box 5114, Port Nelson 7043, Nelson, New Zealand;

    Plant Functional Biology & Climate Change Cluster, University Of Technology Sydney, Ultimo, NSW 2007, Australia,Sydney Institute of Marine Sciences, Mosman, NSW 2088, Australia;

    School of Biotechnology & Biomolecular Sciences, University of New South Wales, Kensington, NSW 2052, Australia;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Ciguatera fish poisoning; Fish feeding; Gambierdiscus; Maitotoxin;

    机译:Ciguatera鱼中毒;喂鱼;Gambierdiscus;毒素;

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