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首页> 外文期刊>Harmful Algae >Non-toxic and toxic subclones obtained from a toxic clonal culture of Alexandrium tamarense (Dinophyceae): Toxicity and molecular biological feature
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Non-toxic and toxic subclones obtained from a toxic clonal culture of Alexandrium tamarense (Dinophyceae): Toxicity and molecular biological feature

机译：从塔玛亚历山大藻（Dinophyceae）的有毒克隆培养物中获得的无毒和无毒亚克隆：毒性和分子生物学特征

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Alexandrium tamarense (Lebour) Taylor strain OF935-AT6 is a rare strain of paralytic shellfish toxin (PST)-producing dinoflagellate, in which non-toxic and toxic cells are found in an approximately 1:1 ratio, isolated in Japan. The non-toxic characteristics of UAT-014-009, an axenic non-toxic subclone of OF935-AT6, have been confirmed at the attomole per cell level. Three out of nine toxic subclones of OF935-AT6 became non-toxic over a relatively short period of time (4-6 years), while the other toxic subclones retained their toxicity and the non-toxic subclones retained to be non-toxic. Two axenic subclones from OF935-AT6, UAT-014-009 (non-toxic) and Axat-2 (toxic) are indistinguishable from one another, and from popularly known A tamarense by rDNA sequence analysis. The most significant difference identified by subtractive hybridization of cDNA pertains to gene fragments homologous with mitochondrial cytochrome c oxidase polypeptide three (cox3) and cytochrome b (cob). Thus, the polymorphism targeting these regions was investigated by comparison of the gene length amplified by PCR using total DNA from other subclones with a range of toxicities. No direct correlation between any allele and toxicity was observed in this study.
机译：tamarense亚历山大（Lebour）泰勒菌株OF935-AT6是一种罕见的产麻痹性贝类毒素（PST）的鞭毛鞭毛藻菌株，在日本分离出的无毒和毒性细胞比例约为1：1。 UAT-014-009是OF935-AT6的一种轴心性无毒亚克隆，已在每个细胞的原子位水平证实了其无毒特征。 OF935-AT6的9个有毒亚克隆中有3个在相对较短的时间段（4-6年）内变为无毒，而其他有毒亚克隆保留了它们的毒性，而无毒亚克隆则保持为无毒。通过rDNA序列分析，来自OF935-AT6的两个轴突亚克隆，UAT-014-009（无毒）和Axat-2（有毒）彼此之间没有区别，与流行的A tamarense也没有区别。通过cDNA的减性杂交鉴定出的最显着差异涉及与线粒体细胞色素c氧化酶多肽3（cox3）和细胞色素b（cob）同源的基因片段。因此，通过比较使用来自其他亚克隆的总DNA具有一定毒性的PCR扩增的基因长度，研究了靶向这些区域的多态性。在该研究中未观察到任何等位基因与毒性之间的直接关系。

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