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Yessotoxin production by Gonyaulax spinifera

机译：棘孢子虫产生的毒素

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摘要

Yessotoxin (YTX) contamination of Greenshell™ mussels (Perna canaliculus) in the Marlborough Sounds, New Zealand, occurred in May 2004 with no known YTX-producing algae present in associated seawater samples. Gonyaulax spinifera, previously considered a non-YTX producer, was isolated from the seawater samples and production of YTX by the cells grown in culture was determined by identification of YTXs by ELISA. The algal identification was based on the type description and cyst form. DNA sequence data for G. spinifera differed from the morphologically similar G. membranacea, G. digitale, and G. cf. spinifera. Protoceratium reticulatum was isolated from the same site as G. spinifera 3 weeks later, but was not a YTX-producer by LC-MS and YTX ELISA analyses. Implications of the discovery that YTX is produced by G. spinifera are that this micro-alga will need to be included in future risk assessments for YTX based on the phytoplankton composition.
机译：2004年5月，新西兰Marlborough Sounds的Greenshell™贻贝（Perna canaliculus）的Yessotoxin（YTX）污染发生，相关海水样品中没有已知的产生YTX的藻类。从海水样品中分离出以前被认为是非YTX产生者的刺桐（Gonyaulax spinifera），并通过ELISA鉴定YTX来确定培养物中生长的细胞产生的YTX。藻类鉴定基于类型描述和囊肿形式。 G. spinifera的DNA序列数据与形态相似的G. membranacea，G。digitale和G. cf.不同。棘藻。 3周后从与棘孢菌相同的位点分离出网状原角藻，但通过LC-MS和YTX ELISA分析不是原产YTX的生产者。 YTX是由棘孢菌产生的发现的暗示是，根据浮游植物组成，该微藻将需要包含在YTX的未来风险评估中。

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