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首页> 外文期刊>Harmful Algae >Production of goniodomin A by the planktonic, chain-forming dinoflagellate Alexandrium monilatum (Howell) Balech isolated from the Gulf Coast of the United States
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Production of goniodomin A by the planktonic, chain-forming dinoflagellate Alexandrium monilatum (Howell) Balech isolated from the Gulf Coast of the United States

机译：从美国墨西哥湾沿岸浮游的链状单鞭毛山形亚历山大藻（Howell）Balech生产goniodomin A

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The chain-forming dinoflagellate Alexandrium monilatum has been reported to be associated with widespread discolored water and increased fish mortality in the Mississippi Sound and off the eastern and western coasts of Florida. Previous studies over the last 60-70 years have determined that A. monilatum produces a harmful substance(s) that is predominantly contained in tne cell mass as exhibited by apparent increased toxicity when the organism cytolyses. The current research in our lab corroborated earlier research demonstrating that A. monilatum produces a lipophilic toxin, unlike its Alexandrium relatives noted for their production of saxitoxin-like toxins. Using sophisticated chemical, chromatographic, and analytical techniques, we have successfully purified and identified the molecular structure of the toxin produced by A. monilatum. We utilized a 500 MHz NMR to carry out a number of experiments (i.e., ~1H, ~(13)C, COSY, HSQC, and HMBC) to unambiguously determine the molecular structure of the toxin. In addition, we report mass analysis of the toxin utilizing electrospray ionization-mass spectrometry (ESI-MS), matrix-assisted laser desorption ionization-time of flight-mass spectrometry (MALDI-TOF-MS), and Q-TOF mass spectral techniques. The toxin is representative of a polyether macrolide with an empirical formula of C_(43)H_(60)O_(12). This toxic compound is shown to be identical to a Japanese tidepool toxin identified as goniodomin A, which is produced by another Alexandrium species.
机译：据报道，在密西西比海峡以及佛罗里达州东部和西部沿海地区，成链的鞭毛甲藻亚历山大藻与水变色和鱼类死亡率增加有关。在过去的60-70年中，先前的研究已经确定，梦游曲霉会产生一种有害物质，这种有害物质主要包含在细胞团中，当这种生物体进行细胞溶解时，毒性会明显增加。我们实验室中的当前研究证实了较早的研究，该研究表明，梦游草产生的是一种亲脂性毒素，这与它的亚历山大氏亲属所产生的类似毒素的毒素不同。使用先进的化学，色谱和分析技术，我们已成功纯化和鉴定了由油曲霉产生的毒素的分子结构。我们利用500 MHz NMR进行了许多实验（即〜1H，〜（13）C，COSY，HSQC和HMBC）来明确确定毒素的分子结构。此外，我们报告了利用电喷雾电离质谱（ESI-MS），基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）和Q-TOF质谱技术对毒素进行的质量分析。毒素代表具有化学式C_（43）H_（60）O_（12）的聚醚大环内酯。该有毒化合物被证明与日本潮池毒素相同，后者被识别为淋菌素A，后者是另一种亚历山大藻产生的。

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