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首页> 外文期刊>Harmful Algae >Evidence for the production of a novel proteinaceous hemolytic exotoxin by dinoflagellate Alexandrium taylori
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Evidence for the production of a novel proteinaceous hemolytic exotoxin by dinoflagellate Alexandrium taylori

机译：甲鞭毛亚历山大藻产生新型蛋白质溶血性外毒素的证据

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摘要

We found that a whole cell suspension of Alexandrium taylori, which is toxic to Anemia, causes species-specific hemolysis against mammalian erythrocytes. Among the erythrocytes tested, rabbit and guinea-pig erythrocytes were highly sensitive, but human, sheep, and cattle erythrocytes were insensitive. The cell-free culture supernatant also showed potent hemolytic activity toward rabbit erythrocytes as seen in whole cell suspension. The hemolytic activity in the culture medium gradually increased with increase in cell number during exponential growth phase, and relatively high activity was maintained even after reaching the death phase. These results suggest that the hemolytic substance is actively released into the medium from A. taylori cells rather than simple leakage from ruptured or dead cells, and a part of them are steadily accumulated in the medium during the algal growth. Chemical characterization with ultrafiltration and trypsin-treatment suggested that the hemolytic substance released into the medium is protein-like compound with molecular weight more than 10,000 Da. The ammonium sulfate precipitated fraction obtained from the cell-free supernatant of A. taylori showed cytotoxic effect on HeLa cells as well as the hemolytic activity in a similar concentration range on a protein content basis. Our results suggest that A. taylori produces a novel proteinaceous hemolytic exotoxin.
机译：我们发现，对贫血有毒的亚历山大泰勒菌的全细胞悬浮液会引起针对哺乳动物红细胞的物种特异性溶血。在所测试的红细胞中，兔子和豚鼠的红细胞高度敏感，而人，绵羊和牛的红细胞则不敏感。如在全细胞悬液中所见，无细胞培养物上清液还显示出对兔红细胞的有效溶血活性。在指数生长期，随着细胞数量的增加，培养基中的溶血活性逐渐增加，甚至在达到死亡阶段后仍保持较高的活性。这些结果表明，溶血物质是从Taylori细胞主动释放到培养基中，而不是从破裂或死细胞中简单泄漏，并且它们的一部分在藻类生长过程中稳定地积累在培养基中。超滤和胰蛋白酶处理的化学表征表明，释放到培养基中的溶血物质是分子量超过10,000 Da的蛋白质样化合物。从Ta.ori的无细胞上清液中获得的硫酸铵沉淀级分显示出对HeLa细胞的细胞毒性作用以及在基于蛋白质含量的相似浓度范围内的溶血活性。我们的结果表明，Taylori产生一种新型的蛋白质溶血性外毒素。

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