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首页> 外文期刊>Harmful Algae >Isolation of an algicidal bacterium and its effects against the harmful-algal-bloom dinoflagellate Prorocentrum donghaiense (Dinophyceae)
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Isolation of an algicidal bacterium and its effects against the harmful-algal-bloom dinoflagellate Prorocentrum donghaiense (Dinophyceae)

机译：杀藻细菌的分离及其对有害藻华双鞭毛虫东海原虫（Dinophyceae）的作用

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摘要

The relationship between algicidal bacteria and harmful-algal-bloom-forming dinoflagellates is understudied and their action modes are largely uncharacterized. In this study, an algicidal bacterium (FDHY-03) was isolated from a bloom of Prorocentrum donghaiense and the characteristics of its action against P. donghaiense was investigated at physiological, molecular, biochemical and cytological levels. 16S rDNA sequence analysis placed this strain in the genus of Alteromonas in the subclass of gamma-proteobacteria. Algicidal activity was detected in the bacterial filtrate, suggesting a secreted algicidal principle from this bacterium. Strain FDHY-03 showed algicidal activity on a broad range of HAB-forming species, but the greatest effect was found on P. donghaiense, which showed 91.7% mortality in 24 h of challenge. Scanning electron microscopic analysis indicated that the megacytic growth zone of P. donghaiense cells was the major target of the algicidal action of FDHY-03. When treated with FDHY-03 culture filtrate, P. donghaiense cell wall polysaccharides decreased steadily, suggesting that the algicidal activity occurred through the digestion of cell wall polysaccharides. To verify this proposition, the expression profile of beta-glucosidase gene in FDHY-03 cultures with or without P. donghaiense cell addition was investigated using reverse-transcription quantitative PCR. The gene expression level increased in the presence of P. donghaiense cells, indicative of beta-glucosidase induction by P. donghaiense and the enzyme's role in this dinoflagellate's demise. This study has isolated a new bacterial strain with a strong algicidal capability, documented its action mode and biochemical mechanism, providing a potential source of bacterial agent to control P. donghaiense blooms.
机译：杀藻细菌和有害藻华形成的甲鞭毛藻之间的关系尚未得到充分研究，其作用方式在很大程度上尚未阐明。在这项研究中，从东海原小球藻的花中分离出了一种杀藻细菌（FDHY-03），并从生理，分子，生化和细胞学水平研究了其对东海假单胞菌的作用特性。 16S rDNA序列分析将该菌株置于γ-变形杆菌亚类的Alteromonas属中。在细菌滤液中检测到杀藻活性，表明该细菌分泌了杀藻原理。 FDHY-03菌株对多种形成HAB的菌种均具有杀藻活性，但对东海疟原虫的作用最大，在攻击后24小时内死亡率为91.7％。扫描电子显微镜分析表明，东海假单胞菌细胞的大细胞生长区是FDHY-03杀藻作用的主要靶标。用FDHY-03培养液处理后，东海疟原虫的细胞壁多糖稳步下降，表明其杀藻活性是通过消化细胞壁多糖而产生的。为了证实这一主张，使用逆转录定量PCR研究了添加或不添加东海假单胞菌的FDHY-03培养物中β-葡萄糖苷酶基因的表达谱。在东海假单胞菌细胞的存在下，基因表达水平增加，表明东海假单胞菌诱导了β-葡萄糖苷酶的存在，以及该酶在该鞭毛鞭毛藻死亡中的作用。这项研究分离出了具有强杀藻能力的新细菌菌株，并记录了其作用模式和生化机制，为控制东海疫霉菌的开花提供了潜在的细菌来源。

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