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首页> 外文期刊>Harmful Algae >Morphology, toxicity and molecular characterization of Gambierdiscus spp. towards risk assessment of ciguatera in south central Cuba
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Morphology, toxicity and molecular characterization of Gambierdiscus spp. towards risk assessment of ciguatera in south central Cuba

机译：Gambierdiscus spp的形态，毒性和分子表征。

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摘要

Ciguatera poisoning is caused by the consumption of reef fish or shellfish that have accumulated ciguatoxins, neurotoxins produced by benthic dinoflagellates of the genera Gambierdiscus or Fukuyoa. Although ciguatera constitutes the primary cause of seafood intoxication in Cuba, very little information is available on the occurrence of ciguatoxins in the marine food web and the causative benthic dinoflagellate species. This study conducted on the south-central coast of Cuba reports the occurrence of Gambierdiscus and Fukuyoa genera and the associated benthic genera Ostreopsis and Prorocentrum. Gambierdiscus/Fukuyoa cells were present at low to moderate abundances depending on the site and month of sampling. This genus was notably higher on Dictyotaceae than on other macrophytes. PCR analysis of field-collected samples revealed the presence of six different Gambierdiscus and one Fukuyoa species, including G. caribaeus, G. carolinianus, G. carpenteri, G. belizeanus, F. ruetzleri, G. silvae, and Gambierdiscus sp. ribotype 2. Only Gambierdiscus excentricus was absent from the eight Gambierdiscus/Fukuyoa species known in the wider Caribbean region. Eleven clonal cultures were established and confirmed by PCR and SEM as being either G. carolinianus or G. caribaeus. Toxin production in each isolate was assessed by a radioligand receptor binding assay and found to be below the assay quantification limit. These novel findings augment the knowledge of the ciguatoxin-source dinoflagellates that are present in Cuba, however further studies are needed to better understand the correlation between their abundance, species-specific toxin production in the environment, and the risk for fish contamination, in order to develop better informed ciguatera risk management strategies.
机译：Ciguatera中毒是由于食用了积聚了Ciguatoxin的礁鱼或贝类引起的，该Ciguatoxin是Gambierdiscus属或Fukuyoa属的底鞭毛鞭毛虫产生的神经毒素。尽管雪茄是古巴海鲜中毒的主要原因，但有关海洋食物网中的雪茄毒素的发生情况和底栖鞭毛鞭毛藻种类的信息很少。在古巴中南部海岸进行的这项研究报告了甘比氏菌属和福库亚属的发生以及相关的底栖动物成骨和原着生。冈比亚双歧杆菌/福冈果细胞的丰度低至中等，具体取决于采样地点和采样月份。该属在白茅科中明显高于其他大型植物。对现场采集的样品进行的PCR分析表明，存在6种不同的冈比亚鞭毛虫和一种福冈亚种，包括加勒比G.caribaeus，G.carolinianus，G.carpenteri，G.belizeanus，F.ruetzleri，G.silvae和Gambierdiscus sp。核糖型2。在更广泛的加勒比海地区已知的8个Gambierdiscus / Fukuyoa物种中仅缺少Gambierdiscus excentricus。建立了11种克隆培养物，并通过PCR和SEM确认是卡罗氏菌或卡氏菌。通过放射性配体受体结合测定法评估每个分离物中的毒素产生，发现其低于测定定量极限。这些新颖的发现增加了对古巴存在的雪茄毒素来源的鞭毛藻类的认识，但是为了进一步了解它们的丰度，环境中特定物种毒素的产生与鱼类污染风险之间的相关性，还需要进一步的研究，以便开发更好的知情的卡瓜塔拉风险管理策略。

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