...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Harmful Algae >Evidence for resting cyst production in the cosmopolitan toxic dinoflagellate Karlodiniwn veneficum and the cyst distribution in the China seas
【24h】

Evidence for resting cyst production in the cosmopolitan toxic dinoflagellate Karlodiniwn veneficum and the cyst distribution in the China seas

机译：在中国大都会毒性丁醇酸盐盐的宿主生产中恢复囊肿的证据和中国海域的囊肿分布

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The naked dinoflagellate Karlodinium veneficum is a cosmopolitan and toxic species that frequently forms harmful algal blooms (HABs) in coastal waters. This species has been intensively studied from multiple aspects including toxicology, toxins, nutrition mode (e.g., mixotrophy, phagotrophy, etc.), blooming dynamics, allelopathy, and behavior, while the mechanisms accounting for its global distribution and possible invasion to new regions have not been investigated. Since the first report of a bloom of this species from the South China Sea in 2003, K. veneficum has been frequently detected in coastal waters of China. While resting cyst has been well documented to play vital roles in the initiation and decline of HABs and in facilitating geographical expansion of HABs species, whether or not K. veneficum forms resting cyst remains an open question. Here, we provide proofs for the resting cyst formation in K. veneficum based on both the observations on the life history of clonal cultures and cyst detections from field sediment. We microscopically observed the mating gametes, gametes in fusion, planozygotes (judged from the two longitudinal flagella and cell morphology such as a larger size), dark brown, thick-walled cysts with smooth surface, and cyst germination. The resting cyst was produced homothallically (i.e. from single clonal culture). We also determined the diploidity of cysts via measuring the copy numbers of the large subunit (LSU) rRNA gene in resting cysts and vegetative cells. The presence of K. veneficum cysts in field sediments was detected via fluorescence in situ hybridization (FISH) using species-specific probes, and further confirmed by single-cell PCR sequencing for the FISH-detected cysts. The distribution and abundance of K. veneficum cysts in the China Seas (Bohai Sea, Yellow Sea, East China Sea, and South China Sea) were mapped using a combined approach of real-time PCR and FISH, and quantified after measuring and taking into account the copy numbers of LSU rRNA gene in vegetative cells and cysts. We found a wide distribution of resting cysts of this organism in the seas of China, but generally with a low abundance in most of the samples (0 to 15 cysts per 32 g of wet sediment for FISH method; 0 to 25 cysts per 32 g of wet sediment for qPCR method). The confirmation of resting cyst production from both the laboratory cultures and field sediments and detection of a wide distribution of cysts in the China coasts in this study provide a possible mechanistic explanation for the frequent recurrences of blooms and the cosmopolitan distribution of K. veneficum. Our work also necessitates both a more intensive investigation on the life history (e.g. germination potential of cysts in the field) and an extensive cyst monitoring in coastal sediments, in order to better understand the general ecology and the bloom dynamics specific to this important species.
机译：赤裸的Dinoflagellate Karlodinium Viseficum是一种常规在沿海水域中形成有害的藻类绽放（Habs）的过多和有毒物种。从包括毒理学，毒素，营养模式（例如，混纺，吞噬等），盛开的动态，化感和行为的多个方面，该物种已经深入研究了多个方面，盛开的动态，化感，同时，核对其全球分布和可能入侵新地区的机制没有被调查。自2003年从南中国海盛开的第一份盛开的报告，K.Veficum经常在中国沿海水域检测到。在休息囊肿的同时，有充分的记录在HAB的启动和衰退以及促进HABS物种的地理扩张中起到重要作用，无论是K.Veficum形式休息囊肿仍然是一个开放的问题。在这里，我们基于对野生群植物培养和囊肿检测的观点来提供K.Veneficum中静静脉形成的证据。我们显微镜地观察了交配的配子，融合中的配子，平整板（从两个纵向鞭毛和细胞形态判断，如较大尺寸的尺寸），深褐色，厚壁囊肿，具有光滑的表面和囊肿萌发。将静止的胱最静止的囊肿（即单克隆培养物）产生。我们还通过测量静止囊肿和营养细胞的大亚基（LSU）RRNA基因的拷贝数来确定囊肿的代号。使用物质特异性探针通过荧光（鱼）原位杂交（鱼）检测野生晶体中的K.Veneficum囊肿，并通过单细胞PCR测序进一步证实鱼类检测到的鱼类囊肿。在中国（渤海，黄海，东海和南海）的K.Veneficum囊肿的分布和丰度使用了实时PCR和鱼类的组合方法映射，并在测量和进入后量化估算营养细胞和囊肿中LSU RRNA基因的拷贝数。我们发现在中国海洋中发现这种生物体休息囊肿的广泛分布，但通常在大多数样品中具有较低的丰度（每32克湿沉积物为鱼类方法0至15瓣;每32克0至25颗囊肿QPCR法的湿沉积物。本研究中，从实验室培养和野生实验室沉积物和检测中国沿海地区的囊肿广泛分布的恢复囊肿的确认为繁殖的盛开和K.Veneficum的多彩分布提供了可能的机制解释。我们的工作还需要更加密集的生命历史调查（例如，野外囊肿的萌发潜力）以及沿海沉积物的广泛囊肿监测，以更好地了解对这一重要物种特异性的一般生态和绽放动态。

著录项

  • 来源
    《Harmful Algae》 |2020年第3期|101788.1-101788.13|共13页
  • 作者

    Liu Yuyang; Hu Zhangxi; Deng Yunyan; Tang Ying Zhong;

  • 作者单位

    Chinese Acad Sci Inst Oceanol CAS Key Lab Marine Ecol & Environm Sci 7 Nanhai Rd Qingdao 266071 Peoples R China|Univ Chinese Acad Sci Beijing 100049 Peoples R China;

    Chinese Acad Sci Inst Oceanol CAS Key Lab Marine Ecol & Environm Sci 7 Nanhai Rd Qingdao 266071 Peoples R China|Qingdao Natl Lab Marine Sci & Technol Lab Marine Ecol & Environm Sci Qingdao 266237 Peoples R China|Chinese Acad Sci Ctr Ocean Megasci Qingdao 266071 Peoples R China;

    Chinese Acad Sci Inst Oceanol CAS Key Lab Marine Ecol & Environm Sci 7 Nanhai Rd Qingdao 266071 Peoples R China|Qingdao Natl Lab Marine Sci & Technol Lab Marine Ecol & Environm Sci Qingdao 266237 Peoples R China|Chinese Acad Sci Ctr Ocean Megasci Qingdao 266071 Peoples R China;

    Chinese Acad Sci Inst Oceanol CAS Key Lab Marine Ecol & Environm Sci 7 Nanhai Rd Qingdao 266071 Peoples R China|Qingdao Natl Lab Marine Sci & Technol Lab Marine Ecol & Environm Sci Qingdao 266237 Peoples R China|Chinese Acad Sci Ctr Ocean Megasci Qingdao 266071 Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Karlodinium veneficum; Resting cyst; Distribution; Fluorescence in situ hybridization (FISH); Cyst mapping;

    机译：Karlodinium Veneficum;休息囊肿;分布;荧光原位杂交（鱼）;囊肿映射;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号