Cloning and Sequencing of Kappa Light Chain Gene of a Mouse Monoclonal Antibody Directed Against Potato Virus Y

Liu Dehu; Chen Sanfeng; Li Gangqiang

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Cloning and Sequencing of Kappa Light Chain Gene of a Mouse Monoclonal Antibody Directed Against Potato Virus Y

机译：马铃薯Y病毒小鼠单克隆抗体Kappa轻链基因的克隆与序列分析。

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A cDNA library was constructed in λgt11 vectors, complementary to the mRNA isolated from a mouse hybridoma raised against potato virus Y(PVY). Thirty cDNA clones were selected from the cDNA library by in situ immunohybridization with goat anti-mouse kappa-chain-specific antibody conjugated to alkaline phosphatase, from which one clone, k6, having the largest insert was characterized by sequence analysis. The result shows that the im-munoglobulin messenger RNA corresponding to k6 is 956 nucleotides in length excluding the poly(A) region, among which 31 bases code for the 5′ non-coding region, 57 for the leader sequence of the protein, 657 for the mature protein and 211 for the 3′ non-coding region. Comparison of deduced amino acid sequences of the protein and other kappa light chains shows that they share a 100% identity in their constant regions (CD and 93. 7% identity in their variable regions (VL). The kappa light chain encoded by k6 is considered to be specific to PVY since only one type of light chain is expressed in the hybridoma.

机译：在λgt11载体中构建了一个cDNA文库，该文库与从针对马铃薯病毒Y（PVY）的小鼠杂交瘤中分离的mRNA互补。通过与缀合碱性磷酸酶的山羊抗小鼠κ链特异性抗体原位免疫杂交，从cDNA文库中选择了30个cDNA克隆，通过序列分析表征了其中一个具有最大插入片段的克隆k6。结果显示，与k6对应的免疫球蛋白信使RNA的长度为956个核苷酸（不包括poly（A）区域），其中5'非编码区编码31个碱基，蛋白质前导序列57个碱基，657个对于成熟蛋白而言，其为3，非编码区为211。蛋白质和其他κ轻链推导的氨基酸序列的比较表明，它们在恒定区（CD和93在可变区（VL）具有100％的同一性。由k6编码的κ轻链是由于杂交瘤中仅表达一种类型的轻链，因此被认为对PVY特异。

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来源
《High Technology Letters》 |1995年第2期|p.103-107|共5页
作者
Liu Dehu; Chen Sanfeng; Li Gangqiang;
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作者单位

Biotechnology Research Center, Chinese Academy of Agricultural Sciences, Beijing 100081, P. R. China;

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原文格式 PDF
正文语种 eng
中图分类工业技术;自然科学总论;
关键词
immunoglobulin; potato virus Y; kappa chain; gene cloning; sequence analysis;

机译：免疫球蛋白;马铃薯Y病毒;κ链;基因克隆;序列分析;

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专利

1. Cloning and Sequencing of Kappa Light Chain Gene of a Mouse Monoclonal Antibody Directed Against Potato Virus Y [J] . 刘德虎高技术通讯：英文版 . 1995,第002期

机译：对马铃薯病毒的小鼠单克隆抗体的克隆链基因的克隆和测序
2. Nested polymerase chain reaction amplification and sequencing analysis of the light-chain and heavy-chain variable regions in the influenza A H1N1 virus hemagglutinin monoclonal antibody gene [J] . H.J. Li, C.Y. Guo, J.Y. Sun, Genetics and Molecular Research . 2014,第2期

机译：甲型H1N1流感病毒血凝素单克隆抗体基因轻链和重链可变区的巢式聚合酶链反应扩增和测序分析
3. V kappa gene family in (Glu60 Ala30 Tyr10)n (GAT)-specific antibodies that express CGAT (or pGAT) public idiotypic specificities. Protein and mRNA sequencing of eight monoclonal V kappa chains. [J] . C Tonnelle, J Rocca-Serra, A Moulin, The Journal of Experomental Medicine . 1983,第5期

机译：（Glu60 Ala30 Tyr10）n（GAT）特异性抗体中的Vκ基因家族，表达CGAT（或pGAT）公共独特型特异性。八个单克隆V kappa链的蛋白质和mRNA测序。
4. Studying the Prevalence of Secondary Light Chains in Research Purpose Monoclonal Antibodies with MS-Based De Novo Protein Sequencing [C] . Zac McDonald, Qixin Liu, Mingjie Xie, Protein Engineering Summit. . 2018

机译：基于MS的MS-Co nogo蛋白测序研究了研究目的中次级轻链的患病率
5. Characterization of orthoreovirus core proteins by sequencing and proteomic analysis of avian reovirus μ proteins and development of α-mammalian reovirus core protein monoclonal antibodies [D] . Noad, Lindsay 2005

机译：通过禽呼肠孤病毒μ蛋白的测序和蛋白质组分析以及α-哺乳动物呼肠孤病毒核心蛋白单克隆抗体的开发来表征正咽病毒核心蛋白
6. V kappa gene family in (Glu60 Ala30 Tyr10)n (GAT)-specific antibodies that express CGAT (or pGAT) public idiotypic specificities. Protein and mRNA sequencing of eight monoclonal V kappa chains [O] . 1983

机译：（Glu60 Ala30 Tyr10）n（GAT）特异性抗体中的V kappa基因家族表达CGAT（或pGAT）公共独特型特异性。八个单克隆V kappa链的蛋白质和mRNA测序
7. V kappa gene family in (Glu60 Ala30 Tyr10)n (GAT)-specific antibodies that express CGAT (or pGAT) public idiotypic specificities. Protein and mRNA sequencing of eight monoclonal V kappa chains [O] . 1983

机译：（Glu60 Ala30 Tyr10）n（GAT）特异性抗体中的V kappa基因家族，表达CGAT（或pGAT）公共独特型特异性。八个单克隆V kappa链的蛋白质和mRNA测序
8. Mouse Monoclonal Antibody Specific for an Allotypic Determinant of the Igh(a) Allele of Murine IgM: Genetic and Functional Analysis of Igh-6a Epitopes Using Anti-IgM Monoclonal Antibodies. [R] . Sieckmann, D. G., Stall, A. M., Subbarao, B. 1991

机译：小鼠单克隆抗体特异性针对Igh（a）小鼠Igm等位基因的同种型决定因素：使用抗Igm单克隆抗体的Igh-6a表位的遗传和功能分析。

Cloning and Sequencing of Kappa Light Chain Gene of a Mouse Monoclonal Antibody Directed Against Potato Virus Y

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