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首页> 外文期刊>ICES Journal of Marine Science >Identification of Girella punctata and G. leonina by PCR-RFLP analysis
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Identification of Girella punctata and G. leonina by PCR-RFLP analysis

机译：PCR-RFLP分析鉴定点点长白蚁和莱昂纳豆

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摘要

Two Girella species, Girella punctata and G. leonina, are sympatric sister species with an extensive overlap in their respective distributions on shallow rocky reefs from Hong Kong to the south of the Japanese Islands. Juveniles of the two species cannot be discriminated easily on the basis of external characters. In this study, after morphological identification of the species, sequencing analysis was carried out for the partial 16S ribosomal RNA gene and for the D-loop region in mitochondrial DNA. A total of 109 specimens were examined. Restriction site mapping of the sequences suggested that the electrophoretic patterns of polymerase chain reaction (PCR)-based restriction fragment length polymorphism (RFLP) analysis of the gene products would produce a species-specific banding pattern. Subsequently, the PCR-RFLP analysis showed that the method was as effective for separating the two morphologically similar species of the genus Girella as the sequencing analysis.
机译：Girella punctata和G. leonina是两种Girella物种，是同胞姐妹物种，在香港到日本群岛南部的浅礁石上，它们的分布广泛重叠。不能根据外在特征轻易区分这两个物种的少年。在这项研究中，对物种进行形态学鉴定后，对部分16S核糖体RNA基因和线粒体DNA中的D环区域进行了测序分析。总共检查了109个标本。序列的限制性酶切定位表明，基于聚合酶链反应（PCR）的限制性片段长度多态性（RFLP）基因产物的电泳图谱将产生物种特异性的条带图谱。随后，PCR-RFLP分析表明该方法与测序分析一样有效地分离了Girella属的两种形态相似的物种。

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