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首页> 外文期刊>ICES Journal of Marine Science >From DNA to biomass: opportunities and challenges in species quantification of bulk fisheries products
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From DNA to biomass: opportunities and challenges in species quantification of bulk fisheries products

机译：从DNA到生物量：物种量化散货产品的机会和挑战

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摘要

Fisheries enforcement relies on visual catch identification and quantification at sea or when landed. Silage (fish dissolved in acid) and fish blocks (block frozen fish) are promising methods for on-board processing and storage of low-value catches. We examined the use of non-destructive sampling and two DNA-based methods, quantitative PCR (qPCR) and metabarcoding, to assess species composition and relative abundance in industrial grade experimental silage and fish blocks. We demonstrate the ability to identify and quantify DNA from fish species in both products. qPCR analysis of small silage samples collected over 21days detected all target control species. DNA from one species (Atlantic wolffish) was consistently overrepresented while, for three species of gadoids (Atlantic cod, haddock and whiting), the DNA content matched input tissue proportions with high accuracy. qPCR and metabarcoding of fish blocks, sampled as run-off water and exterior swabs, provided consistent species detection, with the highest variance observed in quantification from swab samples. Our analysis shows that DNA-based methods have significant potential as a tool for species identification and quantification of complex on-board-processed seafood products and are readily applicable to taxonomically and morphologically similar fish. There is, however, a need for establishing DNA/weight calibration factors for primary fisheries species.
机译：渔业执法依赖于海上的视觉捕捉和量化或降落时。青贮饲料（溶于酸中的鱼）和鱼块（嵌段冷冻鱼）是用于载机加工和储存低值捕获的有希望的方法。我们研究了使用非破坏性取样和两种基于DNA的方法，定量PCR（QPCR）和成立，以评估物种组成和在工业级实验青贮和鱼块中的相对丰富。我们证明了能够在两种产品中识别和量化DNA的DNA。在21天收集的小型青贮样的QPCR分析检测到所有目标控制物种。来自一个物种（大西洋狼）的DNA始终如一的是，对于三种毒物（大西洋鳕鱼，黑皮鳕和Whiting），DNA含量匹配具有高精度的输入组织比例。 QPCR和对鱼块的沟通，作为径流水和外部拭子采样，提供一致的物种检测，具有从拭子样品定量的最高方差。我们的分析表明，基于DNA的方法具有作为物种识别和量化的综合加工海产品的工具的潜力，并且随时适用于分类和形态学上类似的鱼类。然而，需要为原代渔业物种建立DNA /重量校准因子。

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  • 来源
    《ICES Journal of Marine Science》 |2020年第8期|2557-2566|共10页
  • 作者

    Hansen Brian Klitgaard; Farrant Gregory Kevin; Ogden Rob; Humble Emily; Olafsdottir Gudbjorg; Bekkevold Dorte; Knudsen Steen Wilhelm; Moller Peter Rask; Nielsen Einar Eg;

  • 作者单位

    Tech Univ Denmark Natl Inst Aquat Resources Sect Marine Living Resources Vejlsovej 39 DK-8600 Silkeborg Denmark;

    Matis Ltd Vinlandsleid 12 IS-113 Reykjavik Iceland;

    TRACE Wildlife Forens Network 16 Corstorphine Hill Ave Edinburgh EH12 6LE Midlothian Scotland|Univ Edinburgh Royal Dick Sch Vet Studies Easter Bush Campus Edinburgh EH25 9RG Midlothian Scotland|Univ Edinburgh Roslin Inst Easter Bush Campus Edinburgh EH25 9RG Midlothian Scotland;

    Univ Edinburgh Royal Dick Sch Vet Studies Easter Bush Campus Edinburgh EH25 9RG Midlothian Scotland|Univ Edinburgh Roslin Inst Easter Bush Campus Edinburgh EH25 9RG Midlothian Scotland;

    Matis Ltd Vinlandsleid 12 IS-113 Reykjavik Iceland;

    Tech Univ Denmark Natl Inst Aquat Resources Sect Marine Living Resources Vejlsovej 39 DK-8600 Silkeborg Denmark;

    Univ Copenhagen Nat Hist Museum Denmark Univ Pk 15 DK-2100 Copenhagen O Denmark|NIVA Denmark Water Res Njalsgade 76 DK-2300 Copenhagen S Denmark;

    Univ Copenhagen Nat Hist Museum Denmark Univ Pk 15 DK-2100 Copenhagen O Denmark;

    Tech Univ Denmark Natl Inst Aquat Resources Sect Marine Living Resources Vejlsovej 39 DK-8600 Silkeborg Denmark;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    DNA quantification; metabarcoding; MinION; multi-species fisheries products; quantitative PCR;

    机译：DNA量化;沟通;矿物;多种渔业产品;定量PCR;

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