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Gold-nanoparticle-assisted oligonucleotide immobilisation for improved DNA detection

机译:金纳米粒子辅助寡核苷酸固定化,可改善DNA检测

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摘要

Colloidal gold nanoparticles are investigated as a potential scaffold for the assisted immobilisation of probe oligonucleotides on silicon surfaces. A preliminary study is devoted to the examination of the immobilisation of DNA-modified gold nanoparticles as a function of time, concentration, salt and pH. The DNA-modified nanoparticles self-assembled onto solid surfaces in a three-dimensional self-assembled architecture. The functionalised surfaces are evaluated in diagnostic assays, where their potential to improve the efficiency of the hybridisation reaction is tested. The system utilising DNA-modified nanoparticles produced an enhancement in the hybridisation efficiency and the sensitivity limit by a factor 10 to 100 as compared to a conventional DNA immobilisation system on a planar surface.
机译:研究了胶体金纳米颗粒作为潜在的支架,用于将探针寡核苷酸辅助固定在硅表面上。初步研究致力于根据时间,浓度,盐和pH值检测DNA修饰的金纳米颗粒的固定化。 DNA修饰的纳米粒子以三维自组装结构自组装到固体表面上。在诊断测定中评估功能化的表面,其中测试了其改善杂交反应效率的潜力。与在平面上的常规DNA固定系统相比,利用DNA修饰的纳米颗粒的系统产生的杂交效率和灵敏度极限提高了10到100倍。

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