首页> 外文期刊>IEE Proceedings. Nanobiotechnology >Infrared up-converting phosphors for bioassays
【24h】

Infrared up-converting phosphors for bioassays

机译:红外上转换荧光粉,用于生物测定

获取原文
获取原文并翻译 | 示例
           

摘要

The development of up-converting phosphor reporter particles has added a powerful tool to modern detection technologies. Carefully constructed phosphor reporters have core-shell structures with surface functional groups suitable for standard bio-conjugations. These reporters are chemically stable, possess the unique property of infrared up-conversion, and are readily detected. In contrast to conventional fluorescent reporters, up-converting phosphor particles do not bleach and allow permanent excitation with simultaneous signal integration. A large anti-Stokes shift (up to 500 nm) separates discrete emission peaks from the infrared excitation source. Along with the unmatched contrast in biological specimens due to the absence of autofluorescence upon infrared excitation, up-converting phosphor technology (UPT) has unique properties for highly-sensitive particle-based assays. The production and characteristics of UPT reporter particles as well as their application in various bioassays is reviewed.
机译:上转换荧光粉报告分子的发展为现代检测技术增加了强大的工具。精心构造的荧光报告分子具有核壳结构,其表面官能团适合于标准生物缀合物。这些报道分子是化学稳定的,具有红外上转换的独特特性,并且易于检测。与传统的荧光报告器相比,上转换的荧光粉颗粒不会漂白,并且允许永久激发并同时进行信号积分。大的反斯托克斯位移(最大500 nm)将离散的发射峰与红外激发源分开。由于没有红外激发时的自发荧光,生物样品中的对比度无与伦比,因此上转换荧光粉技术(UPT)对于基于高灵敏度颗粒的测定具有独特的性能。综述了UPT报告基因颗粒的生产和特性,以及它们在各种生物测定中的应用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号