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首页> 外文期刊>Selected Topics in Quantum Electronics, IEEE Journal of >Investigation of Cell Culturing on High-Aspect-Ratio, Three-Dimensional Silicon Microstructures
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Investigation of Cell Culturing on High-Aspect-Ratio, Three-Dimensional Silicon Microstructures

机译：高纵横比三维硅微结构上细胞培养的研究

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In this paper, we show that vertical, high-aspect-ratio 1-D photonic crystals (PhCs), consisting of periodic arrays of 3-μm-thick silicon walls separated by 5- μm-wide gaps with depth of 50 μ m (U-grooves), fabricated by electrochemical micromachining, can be combined with mesenchymal cells directly grown into the gaps. Silicon micromachined dice incorporating side-by-side regions with 2- and 3-D structures, namely, flat silicon, shallow (1–2 μm deep) V-grooves, and high-aspect-ratio U-grooves were used as microincubators for culturing SW613-B3, HeLa, SW480, and MRC-5V1 cell lines. Fluorescence microscopy images, obtained after cell fixation, highlight that all the tested cell lines, characterized by different morphologies, are able to grow and proliferate on 2-D microstructures as well as on the “extreme” environment of the PhC structure. More important, SW480 and MRC-5V1 cell lines exhibit the peculiar ability to penetrate into the PhC structure, extending their body deeply in the narrow gaps between adjacent silicon walls, and to grow attached to the vertical surfaces of such 3-D microstructures. This last result represents a first significant step toward the realization of a new class of cell-based biosensors, exploiting cells as bioreceptors and high-aspect-ratio PhCs as transducers, for label-free optical detection of cellular activities involving changes in cell morphology and/or adhesion.
机译：在本文中，我们显示了垂直的高纵横比一维光子晶体（PhC），该晶体由3μm厚的硅壁的周期性阵列组成，硅壁被5μm宽的间隙隔开，深度为50μm（通过电化学微机械加工制造的U型槽可以与直接生长到间隙中的间充质细胞结合。硅微机械切块结合了具有二维和3-D结构的并排区域，即扁平硅，浅（深1–2μm深）V形槽和高纵横比U形槽作为微孵化器，用于培养SW613-B3，HeLa，SW480和MRC-5V1细胞系。细胞固定后获得的荧光显微镜图像突出表明，所有测试的细胞系均具有不同的形态，能够在二维微结构以及PhC结构的“极端”环境下生长和增殖。更重要的是，SW480和MRC-5V1细胞系具有独特的能力，可以渗透到PhC结构中，在相邻硅壁之间的狭窄缝隙中深深地延伸其身体，并生长附着在此类3-D微结构的垂直表面上。最后的结果代表了实现新型基于细胞的生物传感器的第一步，这是将细胞用作生物受体，将高纵横比的PhC用作传感器的无标记光学检测涉及细胞形态和形态变化的细胞活动的第一步。 /或附着力。

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