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Improved Detection Sensitivity of Line-Scanning Optical Coherence Microscopy

机译:线扫描光学相干显微镜的改进的检测灵敏度

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摘要

Optical coherence microscopy (OCM) is a promising technology for high-resolution cellular-level imaging in human tissues. Line-scanning OCM is a new form of OCM that utilizes line-field illumination for parallel detection. In this study, we demonstrate improved detection sensitivity by using an achromatic design for line-field generation. This system operates at 830-nm wavelength with 82-nm bandwidth. The measured axial resolution is 3.9 μm in air (corresponding to ∼2.9 μm in tissue), and the transverse resolutions are 2.1 μm along the line-field illumination direction and 1.7 μm perpendicular to line illumination direction. The measured sensitivity is 98 dB with 25 line averages, resulting in an imaging speed of ∼2 frames/s (516 lines/s). Real-time, cellular-level imaging of scattering tissues is demonstrated using human-colon specimens.
机译:光学相干显微镜(OCM)是用于人类组织中高分辨率细胞水平成像的有前途的技术。线扫描OCM是OCM的一种新形式,它利用线场照明进行并行检测。在这项研究中,我们证明了通过使用消色差设计产生线场可以提高检测灵敏度。该系统在830nm波长和82nm带宽下运行。在空气中测得的轴向分辨率为3.9μm(相当于组织中的〜2.9μm),沿线场照明方向的横向分辨率为2.1μm,垂直于线照明方向的横向分辨率为1.7μm。测得的灵敏度为98 dB,平均线数为25,因此成像速度约为2帧/秒(516线/秒)。实时的细胞水平的散射组织成像显示使用人类结肠标本。

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