• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control >Microbubble Composition and Preparation for High-Frequency Contrast-Enhanced Ultrasound Imaging: In Vitro and In Vivo Evaluation
【24h】

Microbubble Composition and Preparation for High-Frequency Contrast-Enhanced Ultrasound Imaging: In Vitro and In Vivo Evaluation

机译：微泡成分和高频对比增强超声成像的制备：体外和体内评估。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Although high-frequency ultrasound imaging is gaining attention in various applications, hardly any ultrasound contrast agents (UCAs) dedicated to such frequencies (>15 MHz) are available for contrast-enhanced ultrasound (CEUS) imaging. Moreover, the composition of the limited commercially available UCAs for high-frequency CEUS (hfCEUS) is largely unknown, while shell properties have been shown to be an important factor for their performance. The aim of our study was to produce UCAs in-house for hfCEUS. Twelve different UCA formulations A-L were made by either sonication or mechanical agitation. The gas core consisted of C4F10 and the main coating lipid was either 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC; A-F formulation) or 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC; G-L formulation). Mechanical agitation resulted in UCAs with smaller microbubbles (number weighted mean diameter ~1 μm ) than sonication (number weighted mean diameter ~2 μm ). UCA formulations with similar size distributions but different main lipid components showed that the DPPC-based UCA formulations had higher nonlinear responses at both the fundamental and subharmonic frequencies in vitro for hfCEUS using the Vevo2100 high-frequency preclinical scanner (FUJIFILM VisualSonics, Inc.). In addition, UCA formulations F (DSPC-based) and L (DPPC-based) that were made by mechanical agitation performed similar in vitro to the commercially available Target-Ready MicroMarker (FUJIFILM VisualSonics, Inc.). UCA formulation F also performed similar to Target-Ready MicroMarker in vivo in pigs with similar mean contrast intensity within the kidney ( n=7 ), but formulation L did not. This is likely due to the lower stability of formulation L in vivo. Our study shows that DSPC-based microbubbles produced by mechanical agitation resulted in small microbubbles with high nonlinear responses suitable for hfCEUS imaging.
机译：尽管高频超声成像在各种应用中引起了人们的注意，但几乎没有任何专用于此类频率（> 15 MHz）的超声造影剂（UCA）可用于造影增强超声（CEUS）成像。此外，用于高频CEUS（hfCEUS）的有限的商用UCA的组成在很大程度上是未知的，而壳的性能已被证明是其性能的重要因素。我们研究的目的是为hfCEUS内部生产UCA。通过超声或机械搅拌制备了十二种不同的UCA制剂A-L。气芯由C4F10组成，主要包衣脂质为1,2-二硬脂酰基-sn-甘油-3-磷酸胆碱（DSPC； AF配方）或1,2-二棕榈酰基-sn-甘油-3-磷酸胆碱（DPPC； GL）公式）。机械搅拌导致UCA具有比超声处理（数量加权平均直径〜2μm）更小的微气泡（数量加权平均直径〜1μm）。具有相似的尺寸分布但主要脂质成分不同的UCA配方表明，使用Vevo2100高频临床前扫描仪（FUJIFILM VisualSonics，Inc.），基于DPPC的UCA配方在hfCEUS的体外基频和亚谐波频率下均具有更高的非线性响应。另外，通过机械搅拌制成的UCA制剂F（基于DSPC）和L（基于DPPC）在体外的性能与市售的Target-Ready MicroMarker（FUJIFILM VisualSonics，Inc.）类似。在猪中，UCA制剂F在体内的表现也与Target-Ready MicroMarker相似，肾脏内的平均对比强度相似（n = 7），但是制剂L没有。这可能是由于制剂L在体内的较低稳定性。我们的研究表明，通过机械搅拌产生的基于DSPC的微气泡会产生具有高非线性响应的小微气泡，适用于hfCEUS成像。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号