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首页> 外文期刊>IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control >Acoustic microscopy: a study of contrast in fresh tissue
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Acoustic microscopy: a study of contrast in fresh tissue

机译：声学显微镜：新鲜组织中对比度的研究

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摘要

The scanning laser acoustic microscope (SLAM), which exhibits a resolution of about 15 /spl mu/m in biological materials, was operated at a frequency of 100 MHz to evaluate the source of image contrast. Specifically, the SLAM's quantitative capabilities yielded the ultrasonic propagation properties of attenuation coefficient and propagation speed of rat brain tissue (white versus gray matter) and these properties mere correlated with tissue constituents and image contrast. The SLAM's image contrast between the two brain layers in both fresh and fixed specimens was analyzed subjectively by an experienced microscopist. It was determined that ethanol fixation decreased the image contrast between the brain layers. Additionally, the propagation speed was the least affected property in the fresh tissue specimens yet increased in both brain layers after fixation whereas the attenuation coefficient of white matter in unfixed brain tissue was higher than that of gray matter. These results indicate that the SLAM's acoustic image contrast is a direct reflection of the difference in attenuation coefficient whereas the propagation speed is not a significant contributor to the image contrast.
机译：扫描激光声学显微镜（SLAM）在生物材料中的分辨率约为15 / spl mu / m，在100 MHz的频率下运行以评估图像对比度的来源。具体而言，SLAM的定量能力产生了大鼠脑组织（白与灰质）的衰减系数和传播速度的超声传播特性，而这些特性仅与组织成分和图像对比度相关。经验丰富的显微镜专家对新鲜和固定样本中两个大脑层之间的SLAM图像对比度进行了主观分析。已确定乙醇固着降低了脑层之间的图像对比度。此外，在固定后，在新的组织标本中传播速度是受影响最小的特性，但在两个脑层中都增加了，而在未固定的脑组织中，白质的衰减系数高于灰质。这些结果表明，SLAM的声像对比度是衰减系数差异的直接反映，而传播速度并不是图像对比度的重要因素。

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