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首页> 外文期刊>IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control >Transcranial Low-Intensity Pulsed Ultrasound Modulates Structural and Functional Synaptic Plasticity in Rat Hippocampus
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Transcranial Low-Intensity Pulsed Ultrasound Modulates Structural and Functional Synaptic Plasticity in Rat Hippocampus

机译：经颅低强度脉冲超声调节大鼠海马的结构和功能突触可塑性

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摘要

Plasticity of synaptic structure and function play an essential role in neuronal development, cognitive functions, and degenerative diseases. Recently, low-intensity pulsed ultrasound (LIPUS) stimulation has been reported as a promising technology for neuromodulation. However, the effect of LIPUS stimulation on the structural and functional synaptic plasticity in rat hippocampus has not yet been addressed. The aim of this study was to investigate whether LIPUS stimulation could affect the dendritic structure, electrophysiological properties, and expression level of glutamate receptors GluN2A, GluN2B, and GluR1 subunits in rat hippocampus. Transcranial LIPUS was delivered to CA1 of the intact hippocampus of rats (n = 40) for 10 days (10 min/day) with the following parameters: fundamental frequency of 0.5 MHz, pulse repetition frequency (PRF) of 500 Hz, peak negative pressure of 0.42 MPa, and I-spta of 360 mW/cm(2). The effect of LIPUS on dendritic structure, electrophysiological properties, and the expression of neurotransmitter receptors was measured using Golgi staining, electrophysiological recording, and western blotting, respectively. Golgi staining and electrophysiological recordings showed that LIPUS stimulation significantly increased the density of dendritic spines (0.72 +/- 0.17 versus 0.94 +/- 0.19 spines/mu m, p < 0.01) and the frequency of spontaneous excitatory postsynaptic current (0.37 +/- 0.14 versus 1.77 +/- 0.37 Hz, p < 0.05) of CA1 hippocampal neurons. Furthermore, the western blotting analysis demonstrated a significant increase in the expression level of GluN2A (p < 0.05). The results illustrated the effect of LIPUS on the dendritic structure, function, and neurotransmitter receptors, which may provide a powerful tool for treating neurodegenerative diseases.
机译：突触结构的可塑性和功能在神经元发育，认知功能和退行性疾病中起重要作用。最近，低强度脉冲超声（Lipus）刺激已被报告为神经调节的有希望的技术。然而，缺血对大鼠海马结构和功能突触可塑性的影响尚未得到解决。本研究的目的是研究脂肪刺激是否会影响大鼠海马在大鼠海马中谷氨酸受体GLUN2A，GLUN2B和GLUR1亚基的树突式结构，电生理学性质和表达水平。用以下参数递送到大鼠完整海马（N = 40）的完整海马的Ca1（10分钟/天）的血管血脂：射击频率为0.5MHz，脉冲重复频率（PRF），500Hz，峰值负压0.42MPa，和360mW / cm（2）的I-SPTA。利用Golgi染色，电生理记录和蛋白质印迹测量唇脂对树突结构，电生理性质和神经递质受体的表达的影响。 Golgi染色和电生理记录表明，脂肪刺激显着增加了树突刺的密度（0.72 +/- 0.17，与0.94 +/- 0.19刺/ mu m，p <0.01）和自发兴奋后突触电流的频率（0.37 +/- 0.14与1.77 +/- 0.37 Hz，P <0.05）Ca1海马神经元。此外，蛋白质印迹分析表明GLUN2A的表达水平显着增加（P <0.05）。结果说明了唇脂对树突结构，功能和神经递质受体的影响，这可能提供一种治疗神经变性疾病的有力工具。

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  • 作者

    Huang Xiaowei; Lin Zhengrong; Wang Kaiyue; Liu Xiufang; Zhou Wei; Meng Long; Huang Jiqing; Yuan Kexin; Niu Lili; Zheng Hairong;

  • 作者单位

    Chinese Acad Sci Shenzhen Inst Adv Technol Shenzhen 518055 Peoples R China|Univ Chinese Acad Sci Shenzhen Coll Adv Technol Shenzhen 518055 Peoples R China;

    Chinese Acad Sci Shenzhen Inst Adv Technol Shenzhen 518055 Peoples R China;

    Chinese Acad Sci Shenzhen Inst Adv Technol Shenzhen 518055 Peoples R China|Zhejiang Univ Interdisciplinary Inst Neurosci & Technol Qiushi Acad Adv Studies Hangzhou 310029 Zhejiang Peoples R China;

    Chinese Acad Sci Shenzhen Inst Adv Technol Shenzhen 518055 Peoples R China;

    Chinese Acad Sci Shenzhen Inst Adv Technol Shenzhen 518055 Peoples R China|Univ Chinese Acad Sci Shenzhen Coll Adv Technol Shenzhen 518055 Peoples R China;

    Chinese Acad Sci Shenzhen Inst Adv Technol Shenzhen 518055 Peoples R China;

    Chinese Acad Sci Shenzhen Inst Adv Technol Shenzhen 518055 Peoples R China;

    Tsinghua Univ Sch Med Dept Biomed Engn Beijing 100084 Peoples R China;

    Chinese Acad Sci Shenzhen Inst Adv Technol Shenzhen 518055 Peoples R China;

    Chinese Acad Sci Shenzhen Inst Adv Technol Shenzhen 518055 Peoples R China;

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  • 正文语种 eng
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  • 关键词

    Hippocampus; noninvasive therapies; synaptic plasticity; transcranial ultrasound; ultrasound neurostimulation;

    机译：海马;非侵入性疗法;突触塑性;经颅超声;超声神经刺激;

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