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Combining culturing and 16S rDNA sequencing to reveal seasonal and room variations of household airborne bacteria and correlative environmental factors in nanjing, southeast china

机译:结合培养和16S rDNA测序,揭示南京南京家用空气细菌及相关环境因素的季节性和室内变化

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摘要

Exposure to bioaerosols poses important health effects on occupants. To elucidateseasonal and room variations of household airborne bacteria, this study investigated30 residential homes during summer and winter throughout Nanjing, SoutheastChina, with a humid subtropical climate. Culturing and 16S rDNA sequencing methodswere combined in this study. Results showed that the community structure andcomposition in the same season but different homes show similarity, however, they inthe same home but in different seasons show a huge difference, with Sphingomonas(25.3%), Clostridium (14.8%), and Pseudomonas (7.6%) being the dominant bacteria insummer, and Pseudomonas (57.1%) was dominant bacteria in winter. Culturable concentrationsof bacteria were also significantly higher in summer (854 ± 425 CFU/m~3)than in winter (231 ± 175 CFU/m~3), but difference by home or room was relativelyminor. More than 80% of culturable bacteria (<4.7 μm) could penetrate into lowerrespiratory tract. The seasonal variations of bacterial community and concentrationswere closely associated with seasonal variations of temperature, humidity, and PM_(2.5).Higher concentrations and larger sizes were observed in the bathroom and kitchen,typically with higher humidity than other rooms.
机译:暴露于生物这溶胶对居住者构成重要的健康影响。阐明本研究调查了季节性空气载体细菌的季节性和房间变化南京南京冬季30家住宅,东南部中国,具有潮湿的亚热带气候。培养和16S rDNA测序方法在这项研究中合并。结果表明,社区结构和同一季节的组成,但不同的家庭表现出相似性,但是,他们在同一个家,但在不同的季节表现出巨大的差异,与鞘玉米粥(25.3%),梭菌(14.8%)和假单胞菌(7.6%)是主要细菌夏季,冬季缉获量(57.1%)是冬季显性细菌。培养浓度夏季细菌也明显高(854±425 CFU / M〜3)比在冬天(231±175 cfu / m〜3),但是家庭或房间的差异相对次要的。超过80%的培养细菌(<4.7μm)可以渗透到较低呼吸道。细菌群落和浓度的季节变异与温度,湿度和PM_(2.5)的季节性变化密切相关。在浴室和厨房中观察到更高的浓度和更大的尺寸,通常比其他房间更高的湿度。

著录项

  • 来源
    《Indoor Air》 |2021年第4期|1095-1108|共14页
  • 作者单位

    School of Energy and Environment Southeast University Nanjing China Engineering Research Center for BuildingEnergy Environment & Equipments Ministry of Education China Department of Mechanical andAerospace Engineering SyracuseUniversity Syracuse USA;

    School of Energy and Environment Southeast University Nanjing China Engineering Research Center for BuildingEnergy Environment & Equipments Ministry of Education China;

    Department of Mechanical andAerospace Engineering SyracuseUniversity Syracuse USA;

    School of Energy and Environment Southeast University Nanjing China Engineering Research Center for BuildingEnergy Environment & Equipments Ministry of Education China;

    School of Energy and Environment Southeast University Nanjing China Engineering Research Center for BuildingEnergy Environment & Equipments Ministry of Education China;

    School of Energy and Environment Southeast University Nanjing China Jiangsu Provincial Key Laboratory ofSolar Energy Science and Technology School of Energy and Environment Southeast University Nanjing China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    airborne bacteria; community composition; culturable concentration; diversity; seasonal variation; size distribution;

    机译:空气中的细菌;社区成分;培养浓度;多样性;季节性变化;尺寸分布;

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