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首页> 外文期刊>Inflammation >Preventive Effects of Valnemulin on Lipopolysaccharide-Induced Acute Lung Injury in Mice
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Preventive Effects of Valnemulin on Lipopolysaccharide-Induced Acute Lung Injury in Mice

机译：缬尼莫林对脂多糖诱导的小鼠急性肺损伤的预防作用

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Valnemulin reportedly regulates inflammatory responses in addition to its in vitro antibacterial activity. In this study, we established a mouse model of lipopolysaccharide (LPS)-induced inflammatory lung injury and investigated the effect of valnemulin (100 mg/kg) on acute lung injury (ALI) 8 h after LPS challenge. We prepared bronchoalveolar lavage fluid (BALF) for measuring protein concentrations, cytokine levels, and superoxidase dismutase (SOD) activity, and collected lungs for assaying wet-to-dry weight (W/D) ratios, myeloperoxidase (MPO) activity, cytokine mRNA expression, and histological change. We found that the pre-administration of valnemulin significantly decreases the W/D ratio of lungs, protein concentrations, and the number of total cells, neutrophils, macrophages, and leukomonocytes, and histologic analysis indicates that valnemulin significantly attenuates tissue injury. Furthermore, valnemulin significantly increases LPS-induced SOD activity in BALF and decreases lung MPO activity as well. In addition, valnemulin also inhibits the production of tumor necrosis factor-α, interleukin-6, and interleukin-1β, which is consistent with mRNA expression in lung. The results showed that valnemulin had a protective effect on LPS-induced ALI in mice.
机译：据报道，Valnemulin不仅具有体外抗菌活性，还调节炎症反应。在这项研究中，我们建立了脂多糖（LPS）引起的炎症性肺损伤的小鼠模型，并研究了脂多糖（LPS）攻击后8 h缬尼莫林（100 mg / kg）对急性肺损伤（ALI）的影响。我们准备了支气管肺泡灌洗液（BALF），用于测量蛋白质浓度，细胞因子水平和超氧化物酶歧化酶（SOD）活性，并收集了肺，用于测定干重比（W / D），髓过氧化物酶（MPO）活性，细胞因子mRNA表达和组织学变化。我们发现，预先给予缬氨菌灵可显着降低肺的W / D比，蛋白质浓度以及总细胞，嗜中性粒细胞，巨噬细胞和白细胞的数量，并且组织学分析表明缬氨菌素可显着减轻组织损伤。此外，缬草胺可显着增加LPS诱导的BALF中SOD活性，并同时降低肺MPO活性。此外，缬草胺还抑制肿瘤坏死因子-α，白介素-6和白介素-1β的产生，这与肺中的mRNA表达一致。结果表明，缬氨菌灵对LPS诱导的小鼠ALI具有保护作用。

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