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首页> 外文期刊>Inflammation >The Profile of Prostate Epithelial Cytokines and its Impact on Sera Prostate Specific Antigen Levels
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The Profile of Prostate Epithelial Cytokines and its Impact on Sera Prostate Specific Antigen Levels

机译：前列腺上皮细胞因子的概况及其对血清前列腺特异性抗原水平的影响

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The aim of this study was determined the expression of pro inflammatory cytokines in prostate epithelial cells. Furthermore, we analysed the relation between these cytokines and sera PSA levels according the three groups: 0–4, 4–20 and >20 ng/mL. The study was carried out in five normal prostate (NP), 27 benign prostate hyperplastic (BPH) and 18 prostate cancer (PC). Immunohistochemical and Western blot analysis was performed. Serum levels of PSA were assayed by Immulite autoanalyser. The western Blotting analysis revealed an immunoexpression of IL-1α, IL-6 and TNFα in BPH and PC. IL-1α, was absent in NP. Immunohistochemical analysis showed significant high optical density to IL-1α and IL-6 in cancer epithelial cells (19.45 ± 3.25 and 26.2 ± 3.19) compared to normal cells (1.73 ± 1.51 and 4.83 ± 2.65). While, TNFα optical densities were not significant in NP (12.03 ± 2.9), BPH (9.87 ± 3.85) and PC (13.34 ± 2.34). The different profiles of cytokines according sera PSA levels showed a high immunoexpression of the profile (IL-6+, IL-1α+) in BPH patients with PSA between 0–4 and 4–20 ng/mL. However, PC patients with sera PSA between 4 and 20 ng/mL, showed a significant high immunoexpression of the profile (IL-6+, IL-1α−). This data demonstrate a locally production of pro-inflammatory cytokines by prostate epithelial cells and a cross talk between PSA and these cytokines in prostate pathologies.
机译：这项研究的目的是确定前列腺上皮细胞中促炎细胞因子的表达。此外，我们根据以下3组分析了这些细胞因子与血清PSA水平之间的关系：0–4、4–20和> 20 ng / mL。这项研究是在五个正常前列腺（NP），27个良性前列腺增生（BPH）和18个前列腺癌（PC）中进行的。进行了免疫组织化学和蛋白质印迹分析。通过Immulite自动分析仪测定血清PSA水平。 Western Blotting分析显示BPH和PC中IL-1α，IL-6和TNFα的免疫表达。 IL-1α在NP中不存在。免疫组织化学分析显示，与正常细胞（1.73±1.51和4.83±2.65）相比，癌症上皮细胞中IL-1α和IL-6的光密度显着较高（19.45±3.25和26.2±3.19）。而NP（12.03±2.9），BPH（9.87±3.85）和PC（13.34±2.34）中的TNFα光密度不显着。根据血清PSA水平的不同，不同的细胞因子谱显示PSA在0–4和4–20 ng / mL之间的BPH患者中该谱的免疫表达较高（IL-6 +，IL-1α+）。但是，血清PSA在4至20 ng / mL之间的PC患者表现出显着的高免疫表达（IL-6 +，IL-1α-）。该数据证明前列腺上皮细胞局部产生促炎性细胞因子，以及前列腺病理中PSA与这些细胞因子之间的串扰。

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