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首页> 外文期刊>International journal of environmental analytical chemistry >Competitive immunoassay for monitoring polybrominated diphenyl ethers in PM_(2.5) in Shanghai
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Competitive immunoassay for monitoring polybrominated diphenyl ethers in PM_(2.5) in Shanghai

机译：上海市PM_（2.5）中多溴联苯醚监测的竞争性免疫分析

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摘要

In this paper, a sensitive biotin-streptavidin (BS)-ELISA was developed for determining the polybrominated diphenyl ethers in PM2.5. For establishing this proposed BS-ELISA, we prepared the biotinylated antibody primarily. And for reducing the background interference, some inFLuencing factors and procedures for this immunoassay were also discussed and optimised. Under the optimal conditions, the IC50=0.53ng/mL; IC10 was 0.002ng/mL; and the results were almost consistent with those using the gas chromatography and electron capture detection (GC-ECD). Less procedures and simpler sample preparation were required for this method compared with the GC-ECD. The results showed that the highest value of BDE-47 concentration occurred in December, which might reFLect the combination of heating and industrial pollution. In our analysis, we studied the Pearson correlations between BDE-47/PM2.5 and gaseous pollutants (such as NO2, SO2, CO, O-3, PM10 and PM2.5). BDE-47 showed a higher correlation with NO2 than that with PM10, PM2.5 and SO2, which implied that the BDE-47 emission process might be accompanied by the emission of NO2. Moreover, during the Spring Festival, the concentration of BDE-47 in PM2.5 decreased significantly, whereas the PM2.5 changed little. This suggested factories and vehicles might be the major contributors to BDE-47 emissions (but not to PM2.5).
机译：本文开发了一种灵敏的生物素-链霉亲和素（BS）-ELISA方法，用于测定PM2.5中的多溴联苯醚。为了建立该提议的BS-ELISA，我们主要制备了生物素化抗体。为了减少背景干扰，还讨论并优化了该免疫测定的一些影响因素和程序。在最佳条件下，IC50 = 0.53ng / mL； IC10为0.002ng / mL。结果几乎与使用气相色谱和电子捕获检测（GC-ECD）的结果一致。与GC-ECD相比，此方法所需的程序更少，样品制备更简单。结果表明，BDE-47的最高浓度发生在12月，这可能反映了供热和工业污染的结合。在我们的分析中，我们研究了BDE-47 / PM2.5与气态污染物（例如NO2，SO2，CO，O-3，PM10和PM2.5）之间的Pearson相关性。 BDE-47与NO2的相关性高于与PM10，PM2.5和SO2的相关性，这表明BDE-47的排放过程可能伴随有NO2的排放。此外，在春节期间，PM2.5中的BDE-47浓度显着下降，而PM2.5的变化很小。这表明工厂和车辆可能是BDE-47排放的主要贡献者（但不是PM2.5的排放者）。

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  • 作者

    Zhang Xiaohan; Zhuang Huisheng; Ma Hui; Ping Xianyin; Ding Qian;

  • 作者单位

    Shanghai Jiao Tong Univ, Sch Environm Sci & Technol, Shanghai, Peoples R China;

    Shanghai Jiao Tong Univ, Sch Environm Sci & Technol, Shanghai, Peoples R China;

    Minhang Environm Monitoring Stn Shanghai, Shanghai, Peoples R China;

    Shanghai Jiao Tong Univ, Sch Environm Sci & Technol, Shanghai, Peoples R China;

    Shanghai Jiao Tong Univ, Sch Environm Sci & Technol, Shanghai, Peoples R China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Immunoassay; PBDEs; PM2,5; air monitoring; gaseous pollutants;

    机译：免疫分析;多溴二苯醚;PM2,5;空气监测;气态污染物;

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