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首页> 外文期刊>International journal of environmental analytical chemistry >Acetylcholinesterase immobilised eggshell membrane-based optical biosensor for organophosphate detection
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Acetylcholinesterase immobilised eggshell membrane-based optical biosensor for organophosphate detection

机译：乙酰胆碱酯酶固定的蛋壳膜基光学生物传感器用于有机磷酸盐检测

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摘要

The development of an optical biosensor for the determination of malathion based on acetylcholinesterase (AChE) inhibition using Ellman's reagent is reported. The AChE has been immobilised onto the eggshell membrane (ESM) using glutaraldehyde as a cross-linking agent. Scanning Electron Microscopic (SEM) studies and Fourier Transformed Infra-Red (FTIR) characterisations have been carried out to affirm the successful immobilisation of AChE onto the ESM. Under optimum conditions, the developed biosensor estimated the pesticide concentration in the range of 0.1-50ng/mL with a limit of detection (LOD) of 0.1ng/mL within 30min. Parameters affecting the biosensor response such as concentration of enzyme, substrate and inhibition time were optimised. The stability and reusability of the AChE/ESM sensor have been observed as 31days at 4 degrees C and two times, respectively.
机译：报道了使用Ellman试剂基于乙酰胆碱酯酶（AChE）抑制作用来测定马拉硫磷的光学生物传感器的开发。已使用戊二醛作为交联剂将AChE固定在蛋壳膜（ESM）上。已经进行了扫描电子显微镜（SEM）研究和傅立叶变换红外（FTIR）表征，以确认AChE已成功固定在ESM上。在最佳条件下，开发的生物传感器在30分钟内估计农药浓度在0.1-50ng / mL范围内，检出限（LOD）为0.1ng / mL。优化了影响生物传感器响应的参数，例如酶的浓度，底物和抑制时间。 AChE / ESM传感器的稳定性和可重复使用性分别在4摄氏度和21天下观察到31天。

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