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首页> 外文期刊>The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry >An optical glucose biosensor with eggshell membrane as an enzyme immobilisation platform
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An optical glucose biosensor with eggshell membrane as an enzyme immobilisation platform

机译：以蛋壳膜为酶固定平台的光学葡萄糖生物传感器

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摘要

An optical glucose biosensor using an enzyme-immobilised eggshell membrane and oxygen-sensitive optode membrane for glucose determiantion has been developed. Glucose oxidase was covalently immobilised on an eggshell membrane with subsequent covering of the surface with an oxygen-sensitive optode membrane. The oxygen-sensitive membrane was constructed from tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) ditetrakis(4-chlorophenyl) borate adsorbed on silica gel particles and dispersed in a silicone rubber film. The glucose biosensor was built by positioning both enzyme-immobilised eggshell and oxygen-sensitive optode membranes in a laboratory-made flow cell. The detection scheme was based on the depletion of dissolved oxygen content upon exposure to glucose solution and the rate change in fluorescence intensity of the oxygen-sensitive membrane was monitored and related to the glucose concentration. The effect of dissolved oxygen content, pH, temperature, phosphate buffer concentration and potential interferents has been studied in detail. The glucose biosensor demonstrated a long shelf-life compared with an enzyme-immobilised cellulose membrane. It has been successsfully applied to the determination of glucose content in a beverage sample.
机译：已经开发了使用固定有酶的蛋壳膜和氧敏感光电二极管膜进行葡萄糖测定的光学葡萄糖生物传感器。将葡萄糖氧化酶共价固定在蛋壳膜上，随后用氧敏感的光电二极管膜覆盖表面。氧敏感性膜由吸附在硅胶颗粒上并分散在硅橡胶膜中的三（4,7-二苯基-1,10-菲咯啉）钌（II）二四（4-氯苯基）硼酸盐构成。葡萄糖生物传感器是通过将固定酶的蛋壳膜和对氧敏感的optode膜都放置在实验室制造的流通池中而构建的。该检测方案基于暴露于葡萄糖溶液后溶解氧含量的消耗，并且监测了氧敏感性膜的荧光强度的速率变化并与葡萄糖浓度相关。已详细研究了溶解氧含量，pH，温度，磷酸盐缓冲液浓度和潜在干扰物的影响。与固定化酶的纤维素膜相比，葡萄糖生物传感器显示出长的保存期限。它已成功地应用于饮料样品中葡萄糖含量的测定。

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