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首页> 外文期刊>International journal of environmental analytical chemistry >Development of an indirect enzyme immunoassay for the determination of thiabendazole in white and red wines
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Development of an indirect enzyme immunoassay for the determination of thiabendazole in white and red wines

机译：间接酶联免疫法测定白葡萄酒和红葡萄酒中噻菌灵的开发

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摘要

A competitive indirect enzyme immunoassay for the determination of fungicide thiabendazole in white and red wine samples was developed. The assay had a detection limit of 0.005ngmL(-1), a dynamic range from 0.01 to 2ngmL(-1), and was precise with intra- and inter-assay coefficient of variation values less than 5% and 8%, respectively. Concerning the sample preparation procedure, it was found that filtration of the wine samples through a 0.45 m pore size polytetrafluoroethylene (PTFE) filter followed by 30 times dilution with the assay buffer eliminated completely matrix interferences for both white and red wines derived from different grape varieties. This was demonstrated by the accurate determination of thiabendazole amounts in spiked wine samples (% recovery 90.6-108). In addition, the results obtained for certain spiked wine samples were in good agreement with those received by an LC-MS/MS method for determination of thiabendazole in wines. Taking into account the dilution factor, the thiabendazole concentrations that could be determined in wines ranged from 0.3 to 60ngmL(-1). The maximum residue limit (MRL) of thiabendazole in wine grapes is 50gkg(-1). Thus, the proposed enzyme immunoassay can be applied for the fast and parallel analysis of a high number of white and red wine samples with minimal sample pre-treatment.
机译：建立了竞争性间接酶免疫测定法，用于测定白葡萄酒和红葡萄酒样品中的杀菌剂噻菌灵。该分析的检测限为0.005ngmL（-1），动态范围为0.01至2ngmL（-1），并且精确度高，且分析内和分析间变异系数值分别小于5％和8％。关于样品制备程序，发现通过0.45 m孔径的聚四氟乙烯（PTFE）过滤器过滤葡萄酒样品，然后用测定缓冲液稀释30倍，可以完全消除源自不同葡萄品种的白葡萄酒和红葡萄酒的基质干扰。准确测定加标葡萄酒样品中的噻菌灵含量（回收率90.6-108）证明了这一点。此外，某些加标葡萄酒样品的结果与LC-MS / MS法测定葡萄酒中噻菌灵的结果吻合良好。考虑到稀释因子，葡萄酒中可测出的噻苯达唑浓度范围为0.3至60ngmL（-1）。葡萄酒中噻菌灵的最大残留限量（MRL）为50gkg（-1）。因此，所提出的酶免疫分析法可用于对大量白葡萄酒和红葡萄酒样品进行快速且平行的分析，而无需进行最少的样品预处理。

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  • 作者

    Tsialla Zoi; Ucles-Moreno Ana; Petrou Panagiota; Fernandez-Alba Amadeo R.; Kakabakos Sotirios E.;

  • 作者单位

    NCSR Demokritos, Inst Nucl & Radiol Sci & Technol Energy & Safety, Immunoassay Immunosensors Lab, Aghia Paraskevi 15310, Greece;

    Univ Almeria, European Union Reference Lab Pesticide Residues F, Agrifood Campus Int Excellence ceiA3, Almera 04120, Spain;

    NCSR Demokritos, Inst Nucl & Radiol Sci & Technol Energy & Safety, Immunoassay Immunosensors Lab, Aghia Paraskevi 15310, Greece;

    Univ Almeria, European Union Reference Lab Pesticide Residues F, Agrifood Campus Int Excellence ceiA3, Almera 04120, Spain;

    NCSR Demokritos, Inst Nucl & Radiol Sci & Technol Energy & Safety, Immunoassay Immunosensors Lab, Aghia Paraskevi 15310, Greece;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    thiabendazole; indirect enzyme immunoassay; wine samples; LC-MS; MS;

    机译：噻苯达唑间接酶联免疫法酒样LC-MS质谱;

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