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首页> 外文期刊>International journal of environmental analytical chemistry >Chemiluminescent enzyme-linked immunosorbent assay on a strip to detect Escherichia coli O157:H7
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Chemiluminescent enzyme-linked immunosorbent assay on a strip to detect Escherichia coli O157:H7

机译：在试纸上进行化学发光酶联免疫吸附试验以检测大肠杆菌O157：H7

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摘要

A fast and sensitive chemiluminescent enzyme-linked immunosorbent assay method to measure pathogenic bacteria, Escherichia coli O157:H7, on immuno-chromatographic membrane was studied. Non-specific binding of proteins on membrane strip was controlled to attain the best performance of immunosensor by optimising the composition of a running buffer. The specificity of the proposed immunostrip was confirmed by conducting experiments for four different microorganisms. A chemiluminescent signal could be successfully generated from a proposed immunostrip sensing system, and a significant change in the chemiluminescent light intensity with the concentration of target microbes was obtained. E. coli O157:H7 could be quantitatively measured in the range of 1.1 × 10~3-1.1 × 10~7 CFU (colony forming units) mL~(-1) within 16min by using the developed chemiluminescent immunostrip.
机译：研究了一种快速灵敏的化学发光酶联免疫吸附法在免疫色谱膜上测定致病菌O157：H7的方法。通过优化运行缓冲液的组成，可以控制膜条上蛋白质的非特异性结合，从而获得最佳的免疫传感器性能。通过对四种不同的微生物进行实验，证实了所提出的免疫条的特异性。可以从提出的免疫条检测系统成功产生化学发光信号，并且获得化学发光强度随目标微生物浓度的显着变化。通过使用开发的化学发光免疫条带，可以在16分钟内以1.1×10〜3-1.1×10〜7 CFU（菌落形成单位）mL〜（-1）定量测量O157：H7大肠杆菌。

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