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首页> 外文期刊>International journal of food science & technology >Characterization of polyphenolic and oligosaccharidic fractions extracted from grape seeds followed by the evaluation of prebiotic activity related to oligosaccharides
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Characterization of polyphenolic and oligosaccharidic fractions extracted from grape seeds followed by the evaluation of prebiotic activity related to oligosaccharides

机译：表征从葡萄籽中提取的多酚和寡糖馏分，然后评估与寡糖有关的益生元活性

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摘要

The study was designed to characterize polyphenolic and oligosaccharidic fractions extracted from grape seeds. Polyphenols and oligosaccharides purification has been realized by using a multistep solid-phase extraction (SPE) (using C-18 cartridge followed by carbograph cartridge). The evaluation of the monomeric flavan-3-ol composition of polymeric chains and their relative mean degree of polymerisation (mDP) using the phloroglucinolysis method, combined with HPLC separation, has been realized. Then, the evaluation of the three different oligosaccharidic fractions as potential functional ingredients with prebiotic activity towards well-known probiotic bacteria, allowing to improve their growth during in vitro fermentation, has been deepened. These oligosaccharidic fractions extracted from seeds showed a significant prebiotic activity on probiotic bacteria such as Lactobacillus acidophilus.
机译：该研究旨在表征从葡萄籽中提取的多酚和寡糖馏分。多酚和低聚糖的纯化已通过使用多步固相萃取（SPE）（使用C-18色谱柱，然后使用碳谱仪色谱柱）实现。已经实现了使用光葡糖酶解法结合HPLC分离对聚合链的单体flavan-3-ol组成及其相对平均聚合度（mDP）的评估。然后，对三种不同的寡糖馏分作为潜在的功能成分的评价已加深，这三种成分对众所周知的益生菌具有益生元活性，从而可以改善它们在体外发酵过程中的生长。从种子中提取的这些寡糖馏分对益生菌（如嗜酸乳杆菌）表现出显着的益生元活性。

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