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首页> 外文期刊>International journal of food science & technology >Rapid detection of Listeria monocytogenes using fluorescence immunochromatographic assay combined with immunomagnetic separation technique
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Rapid detection of Listeria monocytogenes using fluorescence immunochromatographic assay combined with immunomagnetic separation technique

机译：利用荧光免疫层测定与免疫磁分离技术的快速检测李斯特菌单核细胞增生

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摘要

To ensure the safety and quality of food ingredients, especially meat and dairy products, a high-throughput, rapid and sensitive method to detect Listeria monocytogenes (LM) is always on a high demand. In this work, a specific induction method to enrich and detect LM based on fluorescence immunochromatographic assay (FICA) combined with immunomagnetic separation (IMS) techniques has been developed. The immunomagnetic-beads (IM) beads were obtained through functionalised magnetic microspheres and the conjugated reaction between the carboxyl on beads surface and amino groups of antibody. The prepared IM-beads could be used for rapidly enriching pathogenic bacteria with fewer steps, resulting in a high specificity for four pathogenic serotypes and about a 40-fold improvement of detection limit compared with FICA only. In addition, this method was successfully applied in LM detection in sausage, pork and milk samples with a potential for further application in rapid on-site detection of pathogenic bacteria.
机译：为确保食品成分的安全性和质量，特别是肉类和乳制品，高通量，快速和敏感的方法检测李斯特菌单核细胞增生（LM）总是在高需求上。在这项工作中，已经开发了一种基于荧光免疫层析测定（FICA）与免疫磁性分离（IMS）技术合并的富集和检测LM的特异性诱导方法。通过官能化磁性微球和羧基在珠子表面和抗体氨基之间的缀合反应获得免疫磁性珠粒（IM）珠粒。制备的IM-珠粒可用于快速富集致病性细菌，其步骤较少，导致四种致病性血清型的高特异性，与FICA相比，对检测极限的约40倍改善。此外，该方法在香肠，猪肉和牛奶样品中成功应用于LM检测，其潜力在致病细菌的快速现场检测中进行进一步应用。

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    Qianru Li; Sai Zhang; Yanxue Cai; Yuexi Yang; Fei Hu; Xiaoyun Liu; Xiaowei He;

  • 作者单位

    College of Food Sciences South China University of Technology Guangzhou 510640 China Fisheries College Guangdong Ocean University Zhanjiang 524088 China;

    National & Local United Engineering Lab of Rapid Diagnostic Test Guangzhou Wondfo Biotech Co. Ltd. Guangzhou 510663 China;

    College of Food Sciences South China University of Technology Guangzhou 510640 China;

    National & Local United Engineering Lab of Rapid Diagnostic Test Guangzhou Wondfo Biotech Co. Ltd. Guangzhou 510663 China;

    National & Local United Engineering Lab of Rapid Diagnostic Test Guangzhou Wondfo Biotech Co. Ltd. Guangzhou 510663 China;

    National & Local United Engineering Lab of Rapid Diagnostic Test Guangzhou Wondfo Biotech Co. Ltd. Guangzhou 510663 China;

    College of Food Sciences South China University of Technology Guangzhou 510640 China;

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  • 正文语种 eng
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  • 关键词

    Fluorescence immunochromatographic; immunomagnetic beads; Listeria monocytogenes; rapid detection;

    机译：荧光免疫层状;免疫磁珠;李斯特菌;快速检测;

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