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Particle recognition in microfluidic applications using a template matching algorithm

机译:使用模板匹配算法的微流体应用中的粒子识别

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We herein examined the ability of a template matching algorithm to recognize particles with diameters ranging from 1 to 20 mu m in a microfluidic channel. The algorithm consisted of measurements of the distance between the templates and the images captured with a high-speed camera in order to search for the presence of the desired particle. The results obtained indicated that the effects of blur and diffraction rings observed around the particle are important phenomena that limit the recognition of a target. Owing to the effects of diffraction rings, the distance between a template and an image is not exclusively linked to the position of the focus plane; it is also linked to the size of the particle being searched for. By using a set of three templates captured at different Z focuses and an 800x magnification, the template matching algorithm has the ability to recognize beads ranging in diameter from 1.7 to 20 mu m with a resolution between 0.3 and 1 mu m. (C) 2016 The Japan Society of Applied Physics
机译:我们在本文中研究了模板匹配算法识别微流体通道中直径范围为1至20微米的颗粒的能力。该算法包括测量模板与用高速相机捕获的图像之间的距离,以便搜索所需粒子的存在。获得的结果表明,在粒子周围观察到的模糊和衍射环的影响是限制目标识别的重要现象。由于衍射环的作用,模板和图像之间的距离并不仅仅与焦平面的位置相关;它也与要搜索的粒子的大小有关。通过使用在不同的Z焦点处捕获的三个模板集和800倍的放大倍率,模板匹配算法具有识别直径范围从1.7到20微米,分辨率在0.3到1微米之间的珠的能力。 (C)2016年日本应用物理学会

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  • 来源
    《Japanese journal of applied physics》 |2016年第6s1期|06GN05.1-06GN05.5|共5页
  • 作者单位

    Kanagawa Acad Sci & Technol, Kawasaki, Kanagawa 2130012, Japan;

    Kanagawa Acad Sci & Technol, Kawasaki, Kanagawa 2130012, Japan|Tokyo Med & Dent Univ, Inst Biomat & Bioengn, Chiyoda Ku, Tokyo 1010062, Japan;

    Kanagawa Acad Sci & Technol, Kawasaki, Kanagawa 2130012, Japan|Tokyo Med & Dent Univ, Inst Biomat & Bioengn, Chiyoda Ku, Tokyo 1010062, Japan;

    Kanagawa Acad Sci & Technol, Kawasaki, Kanagawa 2130012, Japan|Tokyo Med & Dent Univ, Inst Biomat & Bioengn, Chiyoda Ku, Tokyo 1010062, Japan;

    Tokyo Med & Dent Univ, Inst Biomat & Bioengn, Chiyoda Ku, Tokyo 1010062, Japan;

    Tokyo Med & Dent Univ, Inst Biomat & Bioengn, Chiyoda Ku, Tokyo 1010062, Japan|Waseda Univ, Fac Sci & Engn, Dept Phys, Shinjuku Ku, Tokyo 1698555, Japan;

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