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首页> 外文期刊>Japanese journal of applied physics >Application of Organosilane Monolayer Template to Quantitative Evaluation of Cancer Cell Adhesive Ability
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Application of Organosilane Monolayer Template to Quantitative Evaluation of Cancer Cell Adhesive Ability

机译：有机硅烷单层模板在癌细胞粘附能力定量评估中的应用

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摘要

The adhesive ability of two human pancreatic cancer cell lines was evaluated using organosilane monolayer templates (OMTs). Using the OMT, the spreading area of adhered cells can be limited, and this enables us to focus on the initial attachment process of adhesion. Moreover, it becomes possible to arrange the cells in an array and to quantitatively evaluate the number of attached cells. The adhesive ability of the cancer cells cultured on the OMT was controlled by adding (-)-epigallocatechin-3-gallate (EGCG), which blocks a receptor that mediates cell adhesion and is overexpressed in cancer cells. Measurement of the relative ability of the cancer cells to attach to the OMT revealed that the ability for attachment decreased with increasing EGCG concentration. The results agreed well with the western blot analysis, indicating that the OMT can potentially be employed to evaluate the adhesive ability of various cancer cells.
机译：使用有机硅烷单层模板（OMT）评估了两种人胰腺癌细胞系的粘附能力。使用OMT，可以限制粘附细胞的扩散区域，这使我们能够专注于粘附的初始附着过程。而且，可以将细胞排列成阵列并定量评估附着细胞的数量。通过添加（-）-epigallocatechin-3-gallate（EGCG），可以控制培养在OMT上的癌细胞的粘附能力，该物质可阻断介导细胞粘附并在癌细胞中过度表达的受体。癌细胞附着于OMT的相对能力的测量表明，附着能力随EGCG浓度的增加而降低。结果与蛋白质印迹分析非常吻合，表明OMT可以潜在地用于评估各种癌细胞的粘附能力。

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  • 来源
    《Japanese journal of applied physics》 |2011年第6issue2期|p.06GL01.1-06GL01.4|共4页
  • 作者

    Takashi Tanii; Kosuke Sasaki; Kota Ichisawa; Takanori Demura; Yuichi Beppu; Hoan Anh Vu; Hoan Thanh Chi; Hideaki Yamamoto; Yuko Sato;

  • 作者单位

    School of Science and Engineering, Waseda University, Shinjuku, Tokyo 169-8555, Japan;

    School of Science and Engineering, Waseda University, Shinjuku, Tokyo 169-8555, Japan;

    School of Science and Engineering, Waseda University, Shinjuku, Tokyo 169-8555, Japan;

    School of Science and Engineering, Waseda University, Shinjuku, Tokyo 169-8555, Japan;

    School of Science and Engineering, Waseda University, Shinjuku, Tokyo 169-8555, Japan;

    School of Science and Engineering, Waseda University, Shinjuku, Tokyo 169-8555, Japan;

    School of Science and Engineering, Waseda University, Shinjuku, Tokyo 169-8555, Japan;

    School of Science and Engineering, Waseda University, Shinjuku, Tokyo 169-8555, Japan;

    Research Institute of National Center for Global Health and Medicine, Shinjuku, Tokyo 162-8602, Japan;

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