Protein Cryocrystallography Using Laser-Processed Crystal

Hiroshi KITANO; Hiroyoshi MATSUMURA; Hiroaki ADACHI; Satoshi MURAKAMI; Kazufumi Takano; Tsuyoshi Inoue; Yusuke Mori; Masaaki Doi; Takatomo Sasaki

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Protein Cryocrystallography Using Laser-Processed Crystal

机译：使用激光加工晶体的蛋白质低温晶体学

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We propose a new procedure in biological cryocrystallography, using the laser-ablation technique. This is the first report on the successful processing of cryo-cooled crystals to create a "protein crystal ball" that is conducive to X-ray diffraction (XRD) data collection. Pulsed UV laser soft ablation (PULSA) modifies protein crystals into a spherical shape and removes surrounding materials, but does not negatively affect crystallinity and may improve diffraction data quality. Additionally, we demonstrate treatment of problematic samples to make them serviceable for XRD analysis. Isolation of single crystals can be performed after flash cooling with the PULSA technique.

机译：我们提出了一种使用激光烧蚀技术的生物冷冻晶体学的新程序。这是有关成功加工低温冷却晶体以创建有助于X射线衍射（XRD）数据收集的“蛋白质晶体球”的第一份报告。脉冲UV激光软烧蚀（PULSA）将蛋白质晶体修改为球形并去除周围的物质，但不会对结晶度产生负面影响，并可以改善衍射数据的质量。此外，我们演示了对有问题的样本的处理，以使其可用于XRD分析。可以使用PULSA技术在闪蒸冷却后进行单晶分离。

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来源
《Japanese Journal of Applied Physics. Part 2, Letters & Express Letters》 |2005年第7期|p.L54-L56|共3页
作者
Hiroshi KITANO; Hiroyoshi MATSUMURA; Hiroaki ADACHI; Satoshi MURAKAMI; Kazufumi Takano; Tsuyoshi Inoue; Yusuke Mori; Masaaki Doi; Takatomo Sasaki;
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作者单位

Department of Electrical Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan;

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原文格式 PDF
正文语种 eng
中图分类应用物理学;
关键词
protein crystal; cryocrystallography; laser ablation; UV laser; X-ray diffraction; structural analysis;

机译：蛋白晶体;低温晶体学;激光烧蚀;紫外激光;X射线衍射;结构分析;

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专利

1. Macromolecular cryocrystallography-methods for cooling and mounting protein crystals at cryogenic temperatures. [J] . Pflugrath JW Methods: A Companion to Methods in Enzymology . 2004,第3期

机译：大分子冷冻晶体学方法，用于在低温下冷却和固定蛋白质晶体。
2. Gentle vapor-diffusion technique for cross-linking of protein crystals for cryocrystallography [J] . Lusty C.J. Journal of Applied Crystallography . 1999,第1期

机译：用于晶体结晶的蛋白质晶体交联的温和蒸汽扩散技术
3. Protein Crystal Growth Using Laser-Processed Seed Crystals [J] . Kengo TAKEUCHI, Hiroshi KITANO, Hiroaki ADACHI, Japanese Journal of Applied Physics. Part 1, Regular Papers & Short Notes . 2005,第5A期

机译：使用激光处理的种子晶体生长蛋白质晶体
4. Conformational Change of Proteins at Calcium Phosphate Apatite Crystals and Its Dependenceon Crystal Maturation in Aqueous Solution [C] . Woo-Kul Lee, Diane Eichert, Christian Rey, Annual meeting of the Society For Biomaterials . 2002

机译：磷酸钙磷灰石晶体蛋白质的构象变化及其对水溶液中晶体成熟度的影响
5. Crystal structures of the Fanconi anemia proteins: Structure of the interstrand cross-linking repair protein Fanconi anemia protein I (FANCI); structure of the human FANCF C-terminal domain; reconstitution and crystallilzation of the sub-complexes in the Fanconi anemia core complex. [D] . Xu, Guozhou. 2009

机译：Fanconi贫血蛋白的晶体结构：链间交联修复蛋白Fanconi贫血蛋白I（FANCI）的结构；人FANCF C末端结构域的结构； Fanconi贫血核心复合物中亚复合物的重组和结晶。
6. Hyperquenching for protein cryocrystallography [O] . Matthew Warkentin, Viatcheslav Berejnov, Naji S. Husseini, -1

机译：蛋白质晶体结晶超淬火
7. Neutron cryocrystallography of proteins [O] . M. P. Blakeley, A. J. Kalb (Gilboa), J. R. Helliwell, 2005

机译：中子蛋白质冷冻机

Protein Cryocrystallography Using Laser-Processed Crystal

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