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首页> 外文期刊>Japanese journal of applied physics >Tapping-mode scanning probe electrospray ionization: fusion of SPM with mass spectrometry
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Tapping-mode scanning probe electrospray ionization: fusion of SPM with mass spectrometry

机译:攻丝模式扫描探头电喷雾电离:具有质谱法的SPM融合

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摘要

Biological tissues are composed of a wide variety of chemical components, in which the amount and distribution of these components change dynamically according to health status. Mass spectrometry imaging (MSI) can be applied to biomedical research because of its ability to measure chemical components in a sample's local area as a mass spectrum and visualize the chemical species distribution in a sample. This study reviewed the development of the tapping-mode scanning probe electrospray ionization (t-SPESI) technique to combine atomic force microscopy and mass spectrometry. t-SPESI uses a vibrating capillary probe to achieve rapid extraction, ionization, and MSI using picoliter solvents. Visualizing the distribution of disease-related components in the tissue was performed in the MSI of biological tissue. Multimodal imaging by t-SPESI was also demonstrated by implementing the optical detection of a probe vibration and the feedback control system into the t-SPESI.
机译:生物组织由各种化学成分组成,其中这些组分的量和分布根据健康状况动态变化。 质谱成像(MSI)可以应用于生物医学研究,因为它能够将样品局部区域中的化学成分作为质谱,并在样品中可视化化学物质分布。 该研究综述了攻丝模式扫描探针电喷雾电离(T-SPESI)技术的开发,以结合原子力显微镜和质谱。 T-SPESI使用振动毛细管探针来实现使用Picoliter溶剂的快速提取,电离和MSI。 在生物组织的MSI中进行可视化组织中的疾病相关组分的分布。 通过将探头振动和反馈控制系统的光学检测实现到T-SPESI,还通过T-SPESI进行多峰成像。

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