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首页> 外文期刊>Journal of Applied Phycology >Purification and characteristics of fucoidanase obtained from Dendryphiella arenaria TM94
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Purification and characteristics of fucoidanase obtained from Dendryphiella arenaria TM94

机译：毛树登革毛菌TM94中岩藻糖苷酶的纯化及特性

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摘要

Dendryphiella arenaria TM94 is an obligate marine fungus. Fucoidanase expressed by TM94 by solid state fermentation was purified. The fermented solid medium was extracted with citric acid buffer, and the extracts were precipitated by acetone and separated on Sephadex G-100 chromatography. The specific fucoidanase activity of purified enzyme was 27-fold than that of the crude enzyme. The recovery of the enzyme was 17.69%. SDS-PAGE was used to identify the purity and the molecular weight of the fucoidanase. A single band appeared on SDS-PAGE gel which suggested that relatively pure fucoidanase has been obtained. The molecular weight of fucoidanase is 180 kDa and the isoelectric point was about pH 4.4. The purified fucoidanase appeared to have the maximum enzymatic activity at pH 6.0. KM and the maximum velocity of the enzyme was 6.56 mg·mL−1 and 6.55 mg·mL−1·min−1 by using fucoidan from Fucus vesiculosus as substrate. The enzyme may be a type of endo-fucoidanase which could hydrolyze high molecular weight fucoidan to low molecular weight fucoidan rather than to fucose.
机译：槟榔树Dendryphiella arenaria TM94是专性海洋真菌。纯化了由TM94通过固态发酵表达的岩藻糖聚糖酶。发酵后的固体培养基用柠檬酸缓冲液萃取，萃取液用丙酮沉淀，并在Sephadex G-100色谱上分离。纯化酶的岩藻糖聚糖酶比活性是粗酶的27倍。酶的回收率为17.69％。 SDS-PAGE用于鉴定岩藻糖聚糖酶的纯度和分子量。 SDS-PAGE凝胶上出现一条单条带，表明已获得相对纯的岩藻糖聚糖酶。岩藻依聚糖酶的分子量为180 kDa，等电点约为pH 4.4。纯化的岩藻糖聚糖酶似乎在pH 6.0时具有最大的酶促活性。 K M ，酶的最大速度为6.56 mg·mL -1 和6.55 mg·mL -1 ·min - 1 通过使用来自墨角藻的岩藻依聚糖为底物。该酶可以是一种内切葡聚糖酶，其可以将高分子量岩藻依聚糖水解为低分子量岩藻依聚糖而不是岩藻糖。

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