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Study of solvent–protein coupling effects by neutron scattering

机译：中子散射研究溶剂-蛋白质的耦合效应

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The present work aims to characterize the dynamical behavior of proteins immersed in bio-preserving liquids and glasses. For this purpose, the protein dUTPase was chosen, while the selected solvents were glycerol, a triol, and some homologous disaccharides, i.e., trehalose, maltose, and sucrose, which are known to be very effective bio-preserving agents. The results highlight that the disaccharides show a slowing down effect on the water dynamics, which is stronger for trehalose than in the case of the other disaccharides. Furthermore, a characterization of the medium which hosts the protein is performed by using an operative definition of fragility based on the mean square displacement extracted by elastic incoherent neutron scattering, which is directly connected to Angell’s kinetic fragility based on the viscosity. Finally, a study of the dynamics of the protein sequestered within the solvents is performed. The result shows that the protein dynamics is coupled with that of the surrounding matrix.
机译：本工作旨在表征浸没在生物保存液体和玻璃中的蛋白质的动力学行为。为此目的，选择了蛋白质dUTPase，而选择的溶剂是甘油，三醇和一些同源的二糖，即海藻糖，麦芽糖和蔗糖，已知它们是非常有效的生物防腐剂。结果表明，二糖显示出对水动力学的减慢效果，对于海藻糖而言，其效果比其他二糖更强。此外，通过基于弹性非相干中子散射提取的均方位移，基于对脆性的有效定义，对承载蛋白质的介质进行表征，该均方根位移与基于粘度的安吉尔动力学脆性直接相关。最后，对隔离在溶剂中的蛋白质的动力学进行了研究。结果表明，蛋白质动力学与周围基质的动力学耦合。

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