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首页> 外文期刊>Journal of Cachexia, Sarcopenia and Muscle >Longitudinal changes in total body creatine pool size and skeletal muscle mass using the D3-creatine dilution method
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Longitudinal changes in total body creatine pool size and skeletal muscle mass using the D3-creatine dilution method

机译：使用D3肌酸稀释法的总肌酸池大小和骨骼肌质量的纵向变化

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Background We recently validated in cross-sectional studies a new method to determine total body creatine pool size and skeletal muscle mass based on D3-creatine dilution from an oral dose and detection of urinary creatinine enrichment by isotope ratio mass spectrometry (IRMS). Routine clinical use of the method in aging and disease will require repeated application of the method, with a more widely available technology than IRMS, to enable determination of change in skeletal muscle mass in longitudinal studies. We therefore adapted the method to liquid chromatography-tandem mass spectrometry (LC-MS/MS) technology, and sought to establish proof of concept for the repeated application of the method in a longitudinal study. Because the turnover of creatine is slow, it was also critical to determine the impact of background enrichment from an initial dose of oral D3-creatine on subsequent, longitudinal measurements of change in muscle mass.
机译：背景技术我们最近在横断面研究中验证了一种新方法，该方法可根据口服剂量的D3-肌酸稀释并通过同位素比质谱（IRMS）检测尿中肌酐富集来确定全身肌酸池大小和骨骼肌质量。该方法在衰老和疾病中的常规临床应用将要求该方法的重复应用，并且使用比IRMS更广泛的技术，以便能够在纵向研究中确定骨骼肌质量的变化。因此，我们使该方法适用于液相色谱-串联质谱分析（LC-MS / MS）技术，并寻求为该方法在纵向研究中的重复应用建立概念验证。由于肌酸的转换很慢，因此从口服D3肌酸的初始剂量确定背景富集对随后纵向测量肌肉质量变化的影响也很关键。

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