Growth and mechaical properties of lysozyme crystals

M. Tachibana; Y. Kobayashi; T. Shimazu

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Growth and mechaical properties of lysozyme crystals

机译：溶菌酶晶体的生长和力学性能

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摘要

Vickers microhardness of protein crystals of hen egg-white lysozyme wasinvestigated by the indentation method. The indenttions were celarly made on he crystal surfacje and no large elastic recovergy of them was observed. The vlaue of the hardness was estimaed to be 0.2 kgf/mm~2 on the crystals just after they were transferred from solution to air. The hardness drastically increasediwth increasing exposure time to air at room temeprature. The maximum hardness was 20 kgf/mm~2, which is almos the same as hose of van der Waals crsyals. The origin of such a change in hardness is explained in terms of the wate contained in protein crsytals.

机译：采用压痕法研究了鸡蛋清溶菌酶蛋白晶体的维氏显微硬度。凹痕是在晶体表面上进行的，没有观察到较大的弹性回复率。晶体从溶液转移到空气后，其硬度值估计为0.2 kgf / mm〜2。在室温下，硬度急剧增加，同时增加了暴露于空气的时间。最大硬度为20 kgf / mm〜2，与范德华管的软管相同。这种硬度变化的原因是由蛋白质囊状体中所含的水解释的。

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来源
《Journal of Crystal Growth》 |1999年第1期|p.661-664|共4页
作者
M. Tachibana; Y. Kobayashi; T. Shimazu;
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作者单位

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收录信息美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);
原文格式 PDF
正文语种 eng
中图分类晶体学;
关键词
Protein crystal; Hen egg-white lysozme; Microhardness; Plastic deformation; Disloction;

机译：蛋白质晶体;母鸡蛋白溶菌酶;显微硬度;塑性变形;错位;

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1. Spiral Growth Can Enhance Both the Normal Growth Rate and Quality of Tetragonal Lysozyme Crystals Grown under a Forced Solution Flow [J] . Hayashi Yuki, Maruyama Mihoko, Yoshimura Masashi, Crystal growth & design . 2015,第5期

机译：螺旋生长可以提高在强制溶液流下生长的四方溶菌酶晶体的正常生长速度和质量。
2. Investigation of complexation of amlodipine with lysozyme and its effect on lysozyme crystal growth [J] . Spectrochimica acta, Part A. Molecular and biomolecular spectroscopy . 2020,第期

机译：用溶菌酶对氨氯嗪络合的研究及其对溶菌酶晶体生长的影响
3. Observation of crystal growth introduction of lysozyme protein crystals by atomic force microscopy [J] . Gvozdev N., Yaminsky I., Rashkovich L. Macromolecular symposia . 2000,第0期

机译：原子力显微镜观察溶菌酶蛋白晶体的晶体生长引入
4. Effects of Pressure on the Growth Kinetics of Orthorhombic Lysozyme Crystals [C] . Y. Suzuki, H. Suto, T. Sawada, International Conference on High Pressure Bioscience and Biotechnology . 2003

机译：压力对正畸溶菌酶晶体生长动力学的影响
5. Analysis of the protein-salt coupled transport: Precision measurements of multicomponent diffusion coefficients for aqueous lysozyme solutions containing chloride salts at 25°C and in conditions relevant for crystal growth. [D] . Annunziata, Onofrio. 2001

机译：蛋白质-盐耦合运输的分析：在25°C和与晶体生长相关的条件下，精确测量含氯化物盐的溶菌酶水溶液的多组分扩散系数。
6. The effect of protein–precipitant interfaces and applied shear on the nucleation and growth of lysozyme crystals [O] . Nuno M. Reis, Dimitri Y. Chirgadze, Tom L. Blundell, -1

机译：蛋白质-沉淀物界面和施加的剪切对溶菌酶晶体成核和生长的影响
7. Crystal growth in a three-phase system: Diffusion and liquid-liquid phase separation in lysozyme crystal growth [O] . Heijna M.C.R., Enckevort W.J.P. van, Vlieg E. 2007

机译：三相系统中的晶体生长：溶菌酶晶体生长中的扩散和液-液相分离
8. Analysis of Monomer Aggregation and Crystal Growth Rates of Lysozyme [R] . Nadarajah, Arunan 1996

机译：溶菌酶单体聚集和晶体生长速率分析

Growth and mechaical properties of lysozyme crystals

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