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首页> 外文期刊>Journal of dairy research >Casein composition and differential translational efficiency of casein transcripts in donkey's milk
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Casein composition and differential translational efficiency of casein transcripts in donkey's milk

机译:酪蛋白牛奶酪蛋白转录物的酪蛋白组成和差异翻译效率

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The amount of the four caseins (alpha(s1), alpha(s2), beta and kappa-CN) in donkey milk was evaluated by Urea-PAGE analysis at pH 8.6, followed by immuno-detection with polyclonal antibodies, coupled to densitometric analysis. The results showed the percentage of each casein in decreasing order: beta (54.28) alpha(s1) (35.59) alpha(s2) (7.19) kappa-CN (2.79). The mRNA quantification of donkey casein transcripts, carried out by RT-qPCR, showed that the average percentage of corresponding gene transcripts (CSN2, CSN1S1, CSN1S2 I and CSN3) was 70.85, 6.28, 14.23 and 8.65, respectively. The observed translation efficiency, assessed as percentage of single milk casein fraction out of single percentage of transcript, was 0.76, 5.66, 0.50 and 0.32, respectively. The analysis of the sequences flanking the start codon, the codon usage frequencies and the coding sequence length might explain, at least in part, the differential transcriptional and translational rate observed among the casein transcripts.
机译:通过PH 8.6的尿素 - 页面分析评估甜菜乳中的四种酪蛋白(α(S1),α(S2),β和κ-cn)的量,然后用多克隆抗体进行免疫检测,偶联至密度计量分析。结果表明,每种酪蛋白的下降顺序:β(54.28)>α(35.59)>α(S2)(7.19)> Kappa-Cn(2.79)。通过RT-QPCR进行的驴酪蛋白转录物的mRNA定量表明,相应的基因转录物(CSN2,CSN1,CSN1S2 I和CSN3)的平均百分比分别为70.85,6.28,14.23和8.65。观察到的翻译效率分别评估为单百分比转录物的单次乳酪蛋白馏分百分比,分别为0.76,5.66,0.50和0.32。侧翼起始密码子的序列的分析,密码子使用频率和编码序列长度可以至少部分地解释酪蛋白转录物之间观察到的差异转录和平移率。

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