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Influence of nutrient availability on in vitro growth of major bovine mastitis pathogens

机译：养分可用性对重大牛乳腺炎病原体体外生长的影响

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摘要

The aim of the present study was to investigate the effects of milk composition changes on the in vitro growth of bovine mastitis pathogens. Nutritional requirements of three major bovine mastitis pathogens Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), and Streptococcus uberis (S. uberis) were investigated in vitro. We used ultra-high temperature (UHT) treated milk with different contents of fat, protein, and carbohydrates to test the influ-ence of the availability of various milk constituents on pathogen growth characteristics. Additionally, the bacterial growth was investigated under experimentally modified nutrient availability by dilution and subsequent supplementation with individual nutrients (carbohy-drates, different nitrogen sources, minerals, and different types of B vitamins) either to milk or to a conventional medium (thioglycolate broth, TB). Varying contents of fat, protein or lactose did not affect bacterial growth with the exception of growth of S. uberis being promoted in protein-enriched milk. The addition of nutrients to diluted whole milk and TB partly revealed different effects, indicating that there are media-specific growth limiting factors after dilution. Supplementation of minerals to diluted milk did not affect growth rates of all studied bacteria. Bacterial growth in diluted whole milk was decreased by the addition of high concentrations of amino acids in S. aureus, and by urea and additional B vitamins in E. coli and S. aureus. The growth rate of S. uberis was increased by the addition of B vitamins to diluted whole milk. The present results demonstrate that growth-limiting nutrients differ among pathogen types. Because reduced bacterial growth was only shown in diluted milk or TB, it is unlikely that alterations in nutrient availability occurring as a consequence of physiological changes of milk composition in the cow's udder would directly affect the susceptibility or course of bovine mastitis.
机译：本研究的目的是探讨牛奶成分对牛乳腺炎病原体体外生长的影响。在体外研究了三个主要牛乳腺炎病原体大肠杆菌（大肠杆菌），金黄色葡萄球菌（S. aureus）和链球菌uberis（S.uberis）的营养需求。我们使用具有不同含量的脂肪，蛋白质和碳水化合物的超高温（UHT）处理的乳，以测试各种乳成分的可用性对病原体生长特性的影响。另外，通过稀释和随后用牛奶或常规培养基（硫代糖甲酸盐肉汤， TB）。除了在富含蛋白质牛奶中促进的蛋白质中促进的S. Uberis的生长，不同的脂肪，蛋白质或乳糖的含量不影响细菌生长。添加营养素以稀释全脂牛奶和TB部分揭示了不同的效果，表明稀释后存在培养基特异性的生长限制因子。补充矿物稀释的乳乳并不影响所有研究的细菌的生长率。通过在大肠杆菌中添加高浓度的氨基酸，并通过尿素和尿布氏菌，通过尿素和尿素的另外的B维生素减少稀释的全牛奶中的细菌生长。通过加入B维生素来稀释全牛奶，增加了uberis的生长速率。本结果表明，生长限制营养物质在病原体类型中不同。由于降低的细菌生长仅在稀释的牛奶或结核中显示，因此由于牛乳房牛奶组合物的生理变化而发生的营养可用性的改变将直接影响牛乳腺炎的易感性或过程。

著录项

  • 来源
    《Journal of dairy research》 |2021年第1期|80-88|共9页
  • 作者

    Remo Sturmlin; Josef J. Gross; Olga Wellnitz; Lea A. Wagner; Camille Monney; Anna Oevermann; Rupert M. Bruckmaier;

  • 作者单位

    Veterinary Physiology Vetsuisse Faculty University of Bern Bern Switzerland;

    Veterinary Physiology Vetsuisse Faculty University of Bern Bern Switzerland;

    Veterinary Physiology Vetsuisse Faculty University of Bern Bern Switzerland;

    Veterinary Physiology Vetsuisse Faculty University of Bern Bern Switzerland;

    Division of Neurological Sciences Vetsuisse Faculty University of Bern Bern Switzerland;

    Division of Neurological Sciences Vetsuisse Faculty University of Bern Bern Switzerland;

    Veterinary Physiology Vetsuisse Faculty University of Bern Bern Switzerland;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Bacterial growth; bovine mastitis; milk composition; nutritional requirements; pathogens;

    机译：细菌生长;牛乳腺炎;牛奶组成;营养需求;病原体;

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