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首页> 外文期刊>Journal of dairy research >Laboratory-based evaluation of a simplified point-of-care test intended to support treatment decisions in non-severe bovine clinical mastitis
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Laboratory-based evaluation of a simplified point-of-care test intended to support treatment decisions in non-severe bovine clinical mastitis

机译：基于实验室的评估，用于支持非严重牛临床乳腺炎的治疗决策的简化护理症的评估

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摘要

To limit the use of antimicrobials in dairy cattle, farmers are increasingly encouraged to adopt targeted treatment decisions based on knowledge of the pathogens causing clinical mastitis (CM), whereby treatment of non-severe CM is generally recommended for gram-positive mastitis but not for gram-negative or culture-negative mastitis. The objectives of this study were to conduct a laboratory-based evaluation of the performance of a simplified slide test as a tool to differentiate gram-positive CM from other cases of CM, and to compare its performance against a commercially available on-farm test that is commonly used in our area (VetoRapid). Test outcomes after 24-48 h incubation were compared to results from bacteriological culture and matrix-assisted laser desorption/ionization time-of-flight mass spectrom-etry (MALDI-ToF MS). Milk samples (n = 156) were obtained from cases of severe and non-severe CM on seven farms and collected by farm personnel. After removal of small numbers of contaminated samples and organisms with unknown species identity, the simplified slide test showed high sensitivity and accuracy (＞80%), similar to the comparator test. For most outcomes of interest (culture positive, Escherichia coli, or gram-positive growth), the specificity of the slide test was higher than the specificity of the comparator test. When considering non-severe cases of CM only, and interpreting detection of gram-positive organisms as indicative of the need for antimicrobial treatment, the simplified test had higher specificity (77.4% v. 60.4%) and higher positive predictive value (79.7% v. 70.0%) than the comparator test and similar sensitivity (83.9% v. 87.5%). The proportion of sampled CM cases, contaminated samples and gram-positive mastitis cases - which affects the positive and negative predictive value, the economic value of diagnostic testing and its potential to reduce antimicrobial use - differed between farms. The simplicity and accuracy of the slide test could make it an attractive tool for farmers to target antimicrobial treatment of non-severe clinical mastitis.
机译：为了限制乳制品抗微生物的使用，越来越多地鼓励农民基于导致临床乳腺炎（CM）的病原体的知识采用有针对性的治疗决策，从而通常推荐用于革兰氏阳性乳腺炎的非严重厘米，但不适用于革兰氏阴性或培养阴性乳腺炎。本研究的目标是对基于实验室的性能进行实验室的简化载玻片试验的性能，以将来自其他CM的其他情况分化的革兰氏阳性Cm，并比较其对农场上市售的性能通常用于我们的地区（vetorapid）。将24-48小时孵育后的试验结果与细菌培养和基质辅助激光解吸/电离飞行时间质谱 - etry（Maldi-Tof MS）进行孵育。在七场农场的严重和非严重厘米的情况下获得牛奶样品（n = 156）并由农场人员收集。除去少量污染的样品和具有未知物种同一性的生物后，简化的载玻片测试显示出高灵敏度和精度（> 80％），类似于比较器测试。对于大多数感兴趣的结果（培养阳性，大肠杆菌或革兰氏阳性生长），滑动试验的特异性高于比较器试验的特异性。当考虑仅对CM的非严重病例，并解释革兰氏阳性生物的检测为指示需要抗微生物处理的需要，简化的测试具有更高的特异性（77.4％v。60.4％）和更高的阳性预测值（79.7％V. 。70.0％）比比较器测试和类似的灵敏度（83.9％v.87.5％）。采样的CM病例，污染样品和革兰氏阳性乳腺炎病例的比例 - 影响正负预测值，诊断测试的经济价值及其减少抗微生物用途的潜力 - 不同于农场。滑动试验的简单性和准确性可以使其成为农民靶向非严重临床乳腺炎的抗菌治疗的有吸引力的工具。

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  • 来源
    《Journal of dairy research》 |2021年第2期|170-175|共6页
  • 作者

    Francisco B. Malcata; P. Theo Pepler; Ruth N. Zadoks; Lorenzo Viora;

  • 作者单位

    Scottish Centre for Production Animal Health and Food Safety School of Veterinary Medicine College of Medical Veterinary and Life Sciences University of Glasgow Bearsden Road Glasgow G61 1QH UK;

    Institute of Biodiversity Animal Health and Comparative Medicine College of Medical Veterinary and Life Sciences University of Glasgow Bearsden Road Glasgow G61 1QH UK;

    Scottish Centre for Production Animal Health and Food Safety School of Veterinary Medicine College of Medical Veterinary and Life Sciences University of Glasgow Bearsden Road Glasgow G61 1QH UK Institute of Biodiversity Animal Health and Comparative Medicine College of Medical Veterinary and Life Sciences University of Glasgow Bearsden Road Glasgow G61 1QH UK Sydney School of Veterinary Science Faculty of Science University of Sydney Camden New South Wales 2570 Australia;

    Scottish Centre for Production Animal Health and Food Safety School of Veterinary Medicine College of Medical Veterinary and Life Sciences University of Glasgow Bearsden Road Glasgow G61 1QH UK;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Antimicrobial use; bovine clinical mastitis; point-of-care test; treatment;

    机译：抗菌用途;牛临床乳腺炎;护理点测试;治疗;

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